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TECNICAS DE BIOLOGÍA MOLECULAR

TECNICAS DE BIOLOGÍA MOLECULAR. ENDONUCLEASAS O ENZIMAS DE RESTRICCIÓN. Generan cortes internos en la molécula de ADN Pueden generar extremos romos o cohesivos. Enzimas de Restricción. Extremos cohesivos. Extremos romos. Clonación y uso de enzimas. Separación de fragmentos.

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TECNICAS DE BIOLOGÍA MOLECULAR

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  1. TECNICAS DE BIOLOGÍA MOLECULAR

  2. ENDONUCLEASASO ENZIMAS DE RESTRICCIÓN • Generan cortes internos en la molécula de ADN • Pueden generar extremos romos o cohesivos

  3. Enzimas de Restricción

  4. Extremos cohesivos

  5. Extremos romos

  6. Clonación y uso de enzimas

  7. Separación de fragmentos • Geles de Agarosa • Geles de poliacrilamida

  8. CLONACIÓN

  9. PCR (reacción en cadena de polimerasa) Componentes de la reacción • Polimerasa • Primers • dNTP’s • Cloruro de magnesio • Agua • Buffer

  10. SOUTHERN BLOT

  11. 1) Blood sample. 2) DNA is extracted from blood cells. 3) DNA is cut into fragments by a restriction enzyme. 4) The DNA fragments are separated into bands during electrophoresis in an agarose gel. 5) The DNA band pattern in the gel is transferred to a nylon membrane. 6) Radioactive DNA probe, designed to recognise specific DNA sequences, is prepared. 7) Probe DNA binds to the specific DNA sequences on the membrane. 8) Excess DNA is washed off, but the DNA pattern is invisible at this stage. 9) X-ray film is placed next to the membrane & exposed to the radioactive DNA. 10) The developed X-ray shows the pattern of DNA bands

  12. SECUENCIACIÓN

  13. VNTR

  14. DNA fingerprinting in Argentina to establish kinship of the 'disappeared', the children, often born in captivity, of parents kidnapped and assassinated by the former military regime there. Here a geneticist uses a multi-channel pipette to prepare samples for the process. A bank of blood samples, taken from all the potential relatives, provides the DNA for comparison with that from the child in question. Fragments of DNA are seperated into bands by electrophoresis in an agarose gel. Every human being produces a unique pattern of DNA bands, hence its potential as a kind of genetic

  15. CLONACIÓN EN ANIMALES

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