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Mitosis Lab

Learn how to use a microscope to observe mitosis in onion root tip cells. Calculate the percentage of cells in each stage and determine the time spent in each phase of the cell cycle.

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Mitosis Lab

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  1. Ocular lens - 10X Objectives - 4X - 10X - 40X Total Magnification x 10X = 40X x 10X = 100X x 10X = 400X Mitosis Lab Diaphragm – adjusts light & heat • How to use the microscope • Turn on – crank dial to 10 • Adjust light with diaphragm • Place slide in corner & release • retractable arm….gently!!!!!! • Using scanning lens (4X), focus • on sample with coarse focus • Change objective (if needed) • Repeat

  2. Mitosis Lab

  3. Mitosis Lab Figure 12.10

  4. Mitosis Lab Table 3.1 • If your observations had not been restricted to the area of the root tip that is actively dividing, how would your results have been different? • Why did we choose to use the onion root tip to study mitosis? • Calculate the percentage of cells in each stage. Consider that it takes (on average) 24 hours (1440 min) for onion roots tip cells to complete the cell cycle. Knowing this, you can calculate the amount of time spent in each phase of the cell cycle from the percentage of cells found in each stage of mitosis. (Recall 90% of time is spent in interphase.) % of cells in stage (decimal) X 144 min = _____ minutes in that stage (mitotic phase) • Draw an example of each stage. = 25 cells

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