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BSc Clinical Genetics My story!

BSc Clinical Genetics My story!. Donna Woodman MTO2. BSc Clinical Genetics. September 2002 – enrolled at Wolverhampton University for BSc Clinical Genetics Course. 4 year course. 1 day per week. 5 days study leave per year. 1 st year modules. Biochemistry Human Form Human Function

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BSc Clinical Genetics My story!

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  1. BSc Clinical GeneticsMy story! Donna Woodman MTO2

  2. BSc Clinical Genetics • September 2002 – enrolled at Wolverhampton University for BSc Clinical Genetics Course. • 4 year course. • 1 day per week. • 5 days study leave per year.

  3. 1st year modules • Biochemistry • Human Form Human Function • Human Health, Disease Prevention and Treatment • Human Physiology • Molecules and Cells in Health and Disease • Professional Studies in Healthcare Science

  4. 2nd year modules • 15 and 30 credit modules. • Biology of Disease • Molecular and Cellular Biosciences (30 credits - year) • Biomedical Science Practicals: Microbiology, Genetics, Biochemistry.

  5. 3rd and 4th year • 1.5 days per week. • 1 day at university and ½ day in lab (Honours Project). • Advanced Topics in Healthcare Science • Evidence-based Practice • Gene Manipulation • Medical Genetics • Biomedical ethics

  6. Honours Project • Compare Hams, Changs’ and Amniomax for Solid Tissue Culture. • ? Difference between media for establishing and maintaining cell growth. • 3 extra flasks were set up on each sample with each of the different media.

  7. Cell counts using a haemocytometer at 12, 15 and 19 days prior to harvest. • Compared mitotic index, length and spreading post harvest.

  8. Results • 43 samples were tested • 14 samples failed to grow in culture • 3 infected. • Preliminary results: Amniomax established and maintained both skin and villi cultures quickest, followed by Changs and then Hams F10 (control) in both skin and villi samples.

  9. Results • Related Measures Analysis of Variance statistical analysis (ANOVA) showed no significant difference between the medias (culturing). • The Friedman statistical test: No significant difference between the media with respect to slide quality. • More samples need to be tested which may then show a significant difference between the three types of media.

  10. Further studies • Set up samples in mixture of media. • Establish cultures in one type of media and then continue to harvest stage in another. • Use different hypotonic. • Use different ratio of fixative. • Use different concentration of BRDU for harvests.

  11. Advantages of part time degree • Obtain a degree whilst continually gaining experience at work. • Gain background knowledge. • Professional development.

  12. Disadvantages • Can disrupt rotas within the lab as university days change each semester. • Hard work!

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