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MICROBIAL GROWTH.

MICROBIAL GROWTH. By Dr. Ijeoma Nkeiruka Otigbuo. PHYSICAL REQUIREMENTS. Temperature:Psychrophiles, mesophiles, thermophiles, and psychrotrophs. pH: Acidophiles, Extreme halophiles, and facultative halophiles. CHEMICAL REQUIREMENTS. Carbon. Nitrogen, Sulfur, and Phosphorus.

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MICROBIAL GROWTH.

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  1. MICROBIAL GROWTH. By Dr. Ijeoma Nkeiruka Otigbuo.

  2. PHYSICAL REQUIREMENTS. • Temperature:Psychrophiles, mesophiles, thermophiles, and psychrotrophs. • pH: Acidophiles, Extreme halophiles, and facultative halophiles.

  3. CHEMICAL REQUIREMENTS. • Carbon. • Nitrogen, Sulfur, and Phosphorus. • Trace Elements. • Oxygen: Obligate aerobes, Facultative anaerobes, Obligate anaerobes, Aerotolerant anaerobes, and Microaerophiles. • Organic Growth Factors.

  4. TOXIC FORMS OF OXYGEN. • Singlet oxygen. • Superoxide free radicals. • The hydroxyl radical.

  5. CULTURE MEDIA. • Chemically Defined Media. • Complex Media. • Anaerobic Growth Media and Methods. • Special Culture Techniques. • Selective and Differential Media. • Enrichment Culture.

  6. OBTAINING PURE CULTURES. • Streak Plate Method.

  7. PRESERVING BACTERIAL CULTURES. • Deep Freezing. • Lyophilization. • Cryopreservation.

  8. GROWTH OF BACTERIAL CULTURES. • Bacterial Division. • Generation Time. • Phases of Growth: Lag, Log,Stationary, and Death.

  9. DIRECT MEASUREMENT OF MICROBIAL GROTW. • Pate Counts. • Serial Dilutions. • Pour Plates and Spread Plates. • Filtration. • Most Probable Number Method. • Direct Microscopic Count.

  10. ESTIMATION OF NUMBERS BY INDIRECT METHODS. • Turbity. • Metabolic Activity. • Dry Weight.

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