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BioMiNT

BioMiNT. Cell Encapsulation for Tissue Engineering. Alok Vij 1 Faculty Mentor: Professor AP Lee 2,3 Graduate Mentor: JS Fisher 2. 1 Department of Materials Science and Engineering, Northwestern University 2 Department of Biomedical Engineering, University of California, Irvine

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BioMiNT

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  1. BioMiNT Cell Encapsulation for Tissue Engineering Alok Vij1 Faculty Mentor: Professor AP Lee2,3 Graduate Mentor: JS Fisher2 1 Department of Materials Science and Engineering, Northwestern University 2 Department of Biomedical Engineering, University of California, Irvine 3 Department of Mechanical and Aeronautical Engineering, University of California, Irvine IM-SURE Symposium

  2. Agenda • Introduction • Materials • Methods • Results • Conclusions IM-SURE Symposium

  3. Cell Encapsulation • Applications • Tissue Engineering Scaffolds • Immunoprotection of Implants • Single-cell assays Images: www.science.org, www.manager-magazin.de IM-SURE Symposium

  4. Agenda • Introduction • Materials • Methods • Results • Conclusions IM-SURE Symposium

  5. Materials • PDMS devices • Alginate • Oleic acid • Calcium-containing polymerizing agent Images: www-biol.paisley.ac.uk, www.babonline.org IM-SURE Symposium

  6. Materials • Alginate • Biocompatible • Non-degradable • Gentle gelation • Easily manipulated Images: www.lsbu.ac.uk IM-SURE Symposium

  7. Agenda • Introduction • Materials • Methods • Results • Conclusions IM-SURE Symposium

  8. Methods • Device Fabrication • Photoresist • Expose • Develop • PDMS cast • Anneal Image modified from http://www.postech.ac.kr IM-SURE Symposium

  9. Alginate/cell suspension Droplet generation In-channel polymerization Bead isolation Bead harvesting Cell culturing Methods IM-SURE Symposium

  10. Agenda • Introduction • Materials • Methods • Results • Conclusions IM-SURE Symposium

  11. Results • Successes: • Even distribution of cells • Bead polymerization • Droplet generation • Bead isolation • Bead harvesting • Work in progress: • Microbead stability • Cell viability Fibroblasts suspended in alginate IM-SURE Symposium

  12. Results • In-channel polymerization IM-SURE Symposium

  13. Droplet Generation Before: Results IM-SURE Symposium

  14. Droplet Generation Before: Results IM-SURE Symposium

  15. Results • Bead separation • Before: IM-SURE Symposium

  16. Results • Bead Harvesting • Before: IM-SURE Symposium

  17. Device Design • Before: IM-SURE Symposium

  18. Device Design • Before: IM-SURE Symposium

  19. Device Design • Before: IM-SURE Symposium

  20. Device Design • Before: IM-SURE Symposium

  21. Device Design • Before: IM-SURE Symposium

  22. Device Design IM-SURE Symposium

  23. Results • Droplet Generation • After: IM-SURE Symposium

  24. Results • Bead Separation • After: IM-SURE Symposium

  25. Results • Bead Harvesting • After: IM-SURE Symposium

  26. Current Work • Bead stability IM-SURE Symposium

  27. Current work • Cell viability assessment IM-SURE Symposium

  28. Agenda • Introduction • Materials • Methods • Results • Conclusions IM-SURE Symposium

  29. Conclusions • Success! • Next steps: • Integrate with other devices • Cell factor delivery • Concentration gradient generators • Cell sorters • Switches • Publish IM-SURE Symposium

  30. Thank you! • Professor Abe Lee • Jeff Fisher • Said Shokair • National Science Foundation Abe Lee Jeff Fisher Said Shokair IM-SURE Symposium

  31. Questions? IM-SURE Symposium

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