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PENGENALAN KULTUR MEDIA BAKTERI

PENGENALAN KULTUR MEDIA BAKTERI. DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS. KULTUR MEDIA BACTERI UNTUK APA ?. APA YANG DIPERLUKAN?. KEBUTUHAN OKSIGEN. TUJUAN MELAKUKAN PENANAMAN. ISOLASI KULTUR MURNI. TIPE MEDIA TANAM. MEDIA CAIR. Kuliah berikut. fuchsine. Gram Staining Technique.

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PENGENALAN KULTUR MEDIA BAKTERI

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  1. PENGENALAN KULTUR MEDIA BAKTERI DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS KULTUR MEDIA BAKTERI-PTS PET

  2. KULTUR MEDIA BACTERI UNTUK APA ? KULTUR MEDIA BAKTERI-PTS PET

  3. APA YANG DIPERLUKAN? KULTUR MEDIA BAKTERI-PTS PET

  4. KEBUTUHAN OKSIGEN KULTUR MEDIA BAKTERI-PTS PET

  5. TUJUAN MELAKUKAN PENANAMAN KULTUR MEDIA BAKTERI-PTS PET

  6. ISOLASI KULTUR MURNI KULTUR MEDIA BAKTERI-PTS PET

  7. TIPE MEDIA TANAM KULTUR MEDIA BAKTERI-PTS PET

  8. KULTUR MEDIA BAKTERI-PTS PET

  9. KULTUR MEDIA BAKTERI-PTS PET

  10. KULTUR MEDIA BAKTERI-PTS PET

  11. MEDIA CAIR KULTUR MEDIA BAKTERI-PTS PET

  12. KULTUR MEDIA BAKTERI-PTS PET

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  20. Kuliah berikut KULTUR MEDIA BAKTERI-PTS PET

  21. KULTUR MEDIA BAKTERI-PTS PET

  22. KULTUR MEDIA BAKTERI-PTS PET

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  24. fuchsine Gram Staining Technique

  25. KULTUR MEDIA BAKTERI-PTS PET

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  43. Selective Media MSA • Goal: To chemically (or physically) suppress unwanted microbes and encourage desired microbes. Mannitol salt agar : selective for halophiles with 7% salt (osmotic challenge) and differential for mannitol fermenters: good for skin bacterial cultures. EMB Agar: kills gram positives with eosin and methylene blue, selective for gram negatives. Differential for lactose fermenters. Good for growing enterics. McConkey Agar: supresses gram positives with crystal violet and bile salts; also differential for EMB MA KULTUR MEDIA BAKTERI-PTS PET Figure 6.9b, c

  44. Differential Media • Distinguish between different species based on a metabolic ability. Blood agar (sheep’s blood) reveals if hemolytic Mannitol salt agar contains the pH sensitive dye phenol red (yellow when acidic) Se Sa KULTUR MEDIA BAKTERI-PTS PET Figure 6.9a

  45. Direct Measurements of Microbial Growth KULTUR MEDIA BAKTERI-PTS PET Figure 6.19

  46. Estimating Bacterial Numbers by Indirect methods • Direct Measures • Plate counts of viable bacterial forming colonies • Counting low viable bacterial numbers by filtration • Counting viable bacteria with Most Probable Number • Counting bacteria per ml in direct microscopy • Indirect Measures • Turbidity/Absorbance with a spectrophotometer • Metabolic activity tracking conversion of colored molecules/enyzme assay • Dry weight by weighing a set volume and knowing weight of one cell KULTUR MEDIA BAKTERI-PTS PET

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