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Engr 1182 Nano Lab

Engr 1182 Nano Lab . Microfluidics. Logistics. Divide your team in half One sub-team do Part A Other sub-team start Part B (cleaning chip/ chipholder ) Teams combine when Part A is done Make sure everyone knows what the other team is doing. Segment A of Lab 2. NOTE: No bubbles

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Engr 1182 Nano Lab

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  1. Engr1182 Nano Lab Microfluidics Presentation Short Title

  2. Logistics • Divide your team in half • One sub-team do Part A • Other sub-team start Part B (cleaning chip/chipholder) • Teams combine when Part A is done • Make sure everyone knows what the other team is doing. Presentation Short Title

  3. Segment A of Lab 2 NOTE: No bubbles in water column. hB hA Step 2 Step 3 Presentation Short Title

  4. Segment B of Lab 2 • Remove the existing PDMS top and acrylic bottom from the chipholder. • Clean the chipholder and new acrylic chip bottom and PDMS lid in the ultrasonic cleaner following the procedure in the video and also described in Supplement 2 of the lab procedure. Follow the procedure carefully! Presentation Short Title

  5. Segment B • Assemble the chipholder and top and bottom using the generic printout for alignment. • Add a drop of water between the bottom of the chipholder and the acrylic chip. This will eliminate air gaps. • Plug unused staging well holes. • Practice pumping fluids with the syringe through the chip to help you design a better chip. • Make sure you have read Supplement 1 on tips for using the chipholder. • DO NOT OVER-TIGHTEN THE NUTS ON THE CHIPHOLDER! Presentation Short Title

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