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MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski

MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski Department of Molecular and Biometric Techniques, Museum and Institute of Zoology, Polish Academy of Science, Warsaw, Poland. Typical organization of mtDNA in animals.

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MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski

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  1. MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski Department of Molecular and Biometric Techniques, Museum and Institute of Zoology, Polish Academy of Science, Warsaw, Poland

  2. Typical organization of mtDNA in animals Biochimica et Biophysica Acta 1819 (2012): 939 - 947

  3. Multipartite mitochondrial DNA Wei D-D, Shao R, Yuan M-L, Dou W, Barker SC, et al. (2012) The Multipartite Mitochondrial Genome of Liposcelis bostrychophila: Insights into the Evolution of Mitochondrial Genomes in Bilateral Animals. PLoS ONE 7(3): e33973. doi:10.1371/journal.pone.0033973

  4. Multipartite mitochondrial DNA Gibson T, Blok VC, Phillips MS, Hong G, Kumarasinghe D, Riley IT, Dowton M (2007) The Mitochondrial Subgenomes of the Nematode Globodera pallida are Mosaics: Evidence of Recombination in an Animal Mitochondrial Genome J Mol Evol 64:463–471

  5. Multipartite mitochondrial DNA Smith DR,Kayal E, Yanagihara AA, Collins AG, Pirro S, Keeling PJ (2011) First Complete Mitochondrial Genome Sequence from a Box Jellyfish Reveals a Highly Fragmented Linear Architecture and Insights into Telomere Evolution Genome Biol. Evol. 4(1):52–58.

  6. Multipartite mitochondrial DNA Shao R, Zhu X-Q, Barker SC, Herd K (2012) Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans Genome Biol. Evol. 4(11):1088–1101

  7. Multipartite mitochondrial DNA Shao R, Zhu X-Q, Barker SC, Herd K (2012) Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans Genome Biol. Evol. 4(11):1088–1101

  8. Multipartite mitochondrial DNA number of minicycles Hexapoda Liposcelis bostrychophila – 2 Damalinia sika – 3 Pediculus humanus – 20 Nematoda Globodera pallida – 7 Globodera rostrochiensis – 7 Rotifera Brachionus plicatilis – 2 Medusozoa Hydra magnipapillata – 2 Mesosoa Dicyema misakiense– 6 Diplonemids Diplonema papillatum > 100

  9. Possible molecular mechanisms responsible for formation and maintaining of multipartite mtDNA • replication - recombination

  10. Kasiviswanathan R, Collins TRL, Copeland WC (2012) The interface of transcription and DNA replication in the mitochondria Biochimica et Biophysica Acta 1819: 970–978

  11. Silencing of TbPIF2 expression resulted in rapid loss of maxicircles with little effect on minicircles Abundance of TbPIF2 is controlled through proteolytic degradation by TbHslVU Klingbeil MM, Shapiro TA (2009) Unraveling the Secrets of RegulatingMitochondrial DNA Replication Molecular Cell 35: 398 - 400

  12. RNA (?) helicase Suv3 Comparison of efficacy of RNA and DNA unwinding reaction mediated by hSuv3p as a function of decreasing enzyme concentration. M. Minczuk, J. Piwowarski, M.A. Papworth, K. Awiszus, S. Schalinski, A. Dziembowski, A. Dmochowska, E. Bartnik, K. Tokatlidis, P.P. Stepien, P. Borowski (2002) Localisation of the human hSuv3p helicase in the mitochondrial matrix and itspreferential unwinding of dsDNA. Nucleic Acids Res. 30: 5074–5086.

  13. A model of the functional interplay between the activities of the yeast mitochondrial degradosome. Szczesny RJ, Borowski LS, Malecki M, Wojcik MA, Stepien PP, Golik P (2012) RNA Degradation in Yeast and Human Mitochondria Biochimica et Biophysica Acta 1819: 1027–1034

  14. Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e16404. doi:10.1371/journal.pone.0016404

  15. Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e16404. doi:10.1371/journal.pone.0016404 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016404

  16. Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e16404. doi:10.1371/journal.pone.0016404 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016404

  17. Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e16404. doi:10.1371/journal.pone.0016404 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016404

  18. Multipartite mitochondrial DNA Shao R, Zhu X-Q, Barker SC, Herd K (2012) Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans Genome Biol. Evol. 4(11):1088–1101

  19. Stem-loop structures in multipartite mtDNA of Globodera GGTTTTTCG T-A T-A T-A T-A T-A T-A T-A T-A Scmt I 5389-5415 GGTTTTTCG A-T A-T A-T A-T A-T A-T A-T A-T A-T Scmt III 1593-1619 TTCTCGGTC T-A T-A T-A T-A T-A A-T A-T T-A Scmt VI 1849-1874 TTAATTAT T-A T-A T-A T-A T-A T-A C-G T-A A-T A-T Scmt VI 2479-2506 TATTCTTTCC T-A A-T A-T A-T A-T A-T A-T T-A Scmt VII 283-308 G. rostrochiensis G. pallida

  20. A B C A B C Scmt I Scmt II Scmt V Scmt III Scmt VI Scmt IV Variations in multipartite mtDNA in populations of Globodera pallida

  21. Thank you for attention

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