LC/MS Chromatography Systems

1. LC/MS Chromatography Systems 212-LC

2. The Complete Solution • Varian LC components, pumps, autosamplers and the • New MS Workstation SW (V6.6) can be used to create • a complete configuration, allowing full control of the • Varian LC/MS system

3. The Complete Solution Software • MS Workstation Software • Single point control of modules and accessories • Effortless detector optimisation and control • Simple navigation while reviewing and processing results • Network compatibility for file management, printing and remote access • Extensive reporting for complete analyses • Fully integrated 212-LC command and control driver

4. The Complete Solution Autosamplers • Autosamplers • Productivity increases with automation of • sample injections. A range of modules are • offered to suit various throughput needs • HTS PAL is a low loss, high speed, high capacity, robust autosampler offering outstanding flexibility and productivity • ProStar 430 is a precise, versatile autosampler delivering reliable results, particularly for limited sample volumes • ProStar 410 is an economical, versitle and easy to use autosampler that is suited to research use, but rugged enough for routing QC work.

5. The Complete Solution HPLC Columns • HPLC Columns • Varianoffers a variety of high quality • columns and media for sample prep • to analytical and on to preparative • applications. • Polaris columns provide unique bonded phases to maximize polar retention and selectivity, while virtually eliminating silonol activity. Polaris sets new standards for peak symmetry, selectivity and reproducibility • Pursuit diphenyl columns deliver fast separations without sacrificing resolution. Moving from analytical development to production – Pursuit materials meet requirements for analytical resolution and high quality prep

6. The Complete Solution HPLC Detectors • HPLC Detectors • Several Varian HPLC detectors • are useful for LC/MS applications • PDA and UV/Vis offer easy offline methods development and trouble shooting for separations • ELSD adds an additional “universal” detection channel to the MS data, finding hidden peaks and giving additional quantitative insights

7. The Complete Solution Chromatograph Pumps • 212-LC Chromatography pump • To meet the flow requirements of modern LC/MS • the use of a binary, low delay volume, high • pressure mixing system is essential. The 212-LC • pumping system has the ability to meet your needs • from fast 4.6 mm columns down to 2 mm columns • for bioseparations. • Quality gradient or blending separations up to 2 mL/min. With excellent accuracy and stability for compositional, flow and pressure • Reliable performance for LC/MS is essential. The mechanical design from the mechanism to no-prime check valves is designed for years of reliable use. • Fully integrated software control under MS Workstation assures complete LC/MS control and data storage

8. The Complete Solution LC Performance – Low Delay Volume • Low delay volume means effective 200 µL/min gradient runs Parabens (Methy, Ethyl, Propyl, Butyl) five minute gradient run, great precision and fast gradient results with little delay volume

9. The Complete Solution LC performance – Compositional Precision • Fine precision of flow and composition make for better • peak ID, retention time accuracy and quantitative quality 10% steps, far less than 1% ripple and accuracy values, 200 µL/min

10. High Performance Low Performance Mid Performance Performance at a Competitive Price Target Position Performance List Price

11. The Complete Solution Benefit Bundles Benefit Bundles: Understanding what customers value

12. The Complete Solution Benefits • Performance • State of Play performance for analytical columns • Quality and Uptime • Modern, conventional reciprocating design for rugged reliable operation • Purchase Price • Strong value for performance, small percentage of the LC/MS package price • Cost of Ownership • ability to run low flows reduces solvent and waste disposal costs • Credibility • Industry standard specifications and performance allows the focus to move to MS benefits and advantages

13. The Complete Solution212-LC • Ordering Information • 0391180001 212-LC • includes 150 µL gradient mixer, heads, plumbing and cables for MS workstation control • Optional Degas strongly recommended for Gradient work • A6313 (4 channel, 1 mL/min) • Optional mixers are available on the price list • Requires MS workstation 6.6 or higher

14. The Complete Solution Pricing • Pricing • Two head design with higher performance and lower delay volume than the 210 series • commands an increased value-price • Minor part of the LC/MS system pricing • 212-LC price is complete • an assembled and working pump for the MS • Pricing on SAP or from local sales managers, approximately 20% increase from 210 s

15. Questions • Can I convert my existing order ? • Shipment: immediately • Only orders with LC/MS will be filled in Q4 • Solo 212-LC orders (for existing MS systems) will ship in Q1 FY07 • New orders should be quoted with 212-LC • If the modules have not been shipped or built, changes can be processed with the associated pricing changes • Other ?