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SEED QUALITY OF MAIZE INBRED LINES WITH DIFFERENT OIL AND PROTEIN CONTENTS AND GENETIC BACKGROUND.

SEED QUALITY OF MAIZE INBRED LINES WITH DIFFERENT OIL AND PROTEIN CONTENTS AND GENETIC BACKGROUND. Miriam Munamava, Susana Goggi and Linda Pollak. Iowa State University and USDA-ARS. Background. Corn breeding program: crossing and selecting for oil and protein.

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SEED QUALITY OF MAIZE INBRED LINES WITH DIFFERENT OIL AND PROTEIN CONTENTS AND GENETIC BACKGROUND.

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  1. SEED QUALITY OF MAIZE INBRED LINES WITH DIFFERENT OIL AND PROTEIN CONTENTS AND GENETIC BACKGROUND. Miriam Munamava, Susana Goggi and Linda Pollak Iowa State University and USDA-ARS

  2. Background • Corn breeding program: crossing and selecting for oil and protein. • Stiff Stalk crossed with germplasm from Argentina. • Non- Stiff Stalk crossed with germplasm from St. Croix. • Developed lines of different oil and protein content.

  3. Objectives • To characterize the inbred lines for seed quality. • To evaluate the effect of oil and protein levels on seed quality. • To evaluate the effect of genetic background and production location on seed quality.

  4. Material and Methods • Seed production Ames-IA Clinton-IL Colombia-MO Hooper-NE Starkville-MS

  5. Entry Seed composition Genotype 1 High protein SC01:N1310-378-11-1-1-7-3-B 2 High protein SC01:N1310-378-11-1-1-4-4-B 3 High protein AR16035:S02-325-1-1-6-4-7-B 4 High protein AR16035:S02-325-1-1-6-6-11-B 5 High oil SC01:N1310-338-31-6-2-1-6-B 6 High oil AR16035:S02-325-1-1-6-6-3-B 7 High oil AR16035:S02-325-1-1-6-3-13-B 8 Low oil AR16035:S02-325-1-1-6-3-9-B 9 B73 10 MO17 Table 1 Description of Germplasm used in the Study

  6. Standard Germination Test Planting on Paper towels Seedling evaluations

  7. Saturated Cold Test Seedsplanting Saturated cold test

  8. Leakage of electrolytes: amino acids and organic acids. Poor membrane integrity in deteriorated, low vigor seeds. Electrical Conductivity Test e- e- e- e-

  9. EC • Imbibe seeds in deionized water for 24 hs at 25o C • Individual Seed Analyzer

  10. AA Test • Increased temperature Temperature: 42o C for 96h • High relative humidity • Planting: Over CCP covered with sand

  11. Other Tests • Soak test • Fast green (FG) • 100 seed weight • Inbred quality index (IQI) SC+AA+FG+Soak + [ 200- (EC6h+EC24h)] 60 IQI =

  12. Results and Discussion

  13. Variable Inbred Inbred*location Standard germination *** *** Cold test ** *** AA ** *** EC *** *** Soak test *** *** Seed weight *** *** Table 2. Levels of significance from ANOVA for ten maize inbred lines evaluated in four locations.

  14. Contrast Cold AA EC Soak AR vs. SC ns ns * * HO vs. LO * ns ns ** B73 vs. AR ns ns ns ns MO17 vs. SC ns ns * ** Table 3. Seed vigor tests comparisons between groups of lines bulked by exotic genetic background, Argentina (AR) and St. Croix (SC), and seed composition, high oil (HO), low oil (LO).

  15. Table 4. Means cold test results Locations IA MO IL NE ..….………………%..…………………. .. 1 HP 60 37 73 61 2 HP 52 73 84 80 3 HP 69 55 52 80 4 HP 77 53 52 68 5 HO 57 37 65 36 6 HO 88 43 39 77 7 HO 75 51 77 71 8 LO 81 59 60 80 9 B73 71 57 77 59 10 MO17 57 65 40 48 LSD 32 22 23 24 (0.05)

  16. Table 5. Means AA test results. Locations IA MO IL NE ..………………..%....……………… 1 HP 37 51 7 7 51 2 HP 41 75 67 60 3 HP 48 64 93 48 4 HP 65 63 56 88 5 HO 43 41 77 35 6 HO 57 63 63 76 7 HO 43 47 87 84 8 LO 45 59 67 53 9 B73 45 68 53 64 10 MO17 56 81 89 61 LSD 32 22 24 26 (0.05)

  17. Locations IA MO IL NE ..…………………… m s ..………………… 1 HP 5.0 7.3 4.6 7.1 2 HP 7.0 3.5 4.5 4.9 3 HP 4.5 4.6 3.0 4.3 4 HP 3.6 5.0 6.5 3.4 5 HO 5.3 3.9 2.1 9.5 6 HO 4.5 3.7 4.2 4.1 7 HO 4.8 3.7 4.0 3.7 8 LO 4.5 5.2 4.2 4.8 9 B73 3.9 2.0 2.3 2.4 10 MO17 3.7 2.6 3.0 4.2 LSD 1.5 2.1 2.0 2.3 (0.05) Table 6 . Means electrical conductivity results (24h).

  18. Table 7 . IQI for different locations. Locations IA MO IL NE 1 5.7 4.4 7.1 5.0 2 4.2 7.1 7.2 6.4 3 6.2 5.2 8.0 6.3 4 8.1 5.0 4.5 7.5 5 4.1 5.4 8.9 1.6 6 7.1 6.5 4.8 6.7 7 5.7 5.4 7.9 7.3 8 6.0 4.4 6. 9 6.2 9 7.0 7.9 8.1 8.0 10 6.6 8.4 8.1 6.0 LSD 1.5 1.8 2.1 2.6 (0.05)

  19. Table 8.IQI and relative ranking of the inbred lines. IQI Ranking 5.4 1 HP 9 6.3 2 HP 5 6.5 3 HP 3 6.3 4 HP 5 5.0 5 HO 10 6.3 6 HO 5 6.6 7 HO 4 5.9 8 LO 8 7.7 9 B73 1 7.2 10 MO17 2

  20. Conclusions • Low protein lines have the lowest seed vigor. When protein levels were high, seed quality was high regardless of oil content. • Mo17 and B73 have the highest IQI. Inbred Quality Index results suggests that seed quality attributes should be considered in a comprehensive breeding program. • Inbred line 4 (high protein) has the highest vigor when produced in IA and NE.

  21. Conclusions • Inbred lines with Argentina germplasm have the highest seed quality. • Seeds produced in IL have the best quality, while those produced in MO have the lowest quality.

  22. Tom Hoegemeyer- NE Kevin Montgomery- IL David Willmot - MO Paul Williams- MS Penny Meyerholz Phillip Dixon (ISU Stats) Acknowledgements

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