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Fluorimetric Analysis

Fluorimetric Analysis. Spectroscopy. Fluorimetric Analysis :

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Fluorimetric Analysis

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  1. Fluorimetric Analysis

  2. Spectroscopy

  3. Fluorimetric Analysis : method of chemical analysis in which a sample, exposed to radiation of one wavelength, absorbs this radiation and reemits radiation of the same or longer wavelength ,the intensity of reemited radiation is almost directly proportional to the concentration of the fluorescing material. Also known as fluorescence analysis; fluorometry.

  4. Comparison

  5. Objectives: 1- Determination of excitation and emission spectra of Quinine SO4. 2- Prepare of calibration curve of Quinine SO4. 3-Determination of Concentration of unk. Quinine SO4 solution. 4-Demonstrate Quenching effect of KI on flourescence of quinine SO4.

  6. Quenching effect What does it mean by Quenching ? Quenching refers to any process which decreases the fluorescence intensity of a given substance. • Quenching may occur by several mechanism: • collisional or dynamic quenching • static quenching • quencing by energy transfer • charge transfer reactions

  7. Quencher

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