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DU145

Figure S1. A. 22Rv1. DU145. PC-3. Relative levels. B. RWPE-1. BPH1. Relative levels. Relative levels. Figure S2. A. RWPE-1. RWPE-1. % Methylated Species. % Methylated Species. B. DU145. DU145. % Methylated Species. % Methylated Species. Figure S3. A. RWPE-1. RWPE-1.

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DU145

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  1. Figure S1 A 22Rv1 DU145 PC-3 Relative levels B RWPE-1 BPH1 Relative levels Relative levels

  2. Figure S2 A RWPE-1 RWPE-1 % Methylated Species % Methylated Species B DU145 DU145 % Methylated Species % Methylated Species

  3. Figure S3 A RWPE-1 RWPE-1 % Methylated Species % Methylated Species B BPH1 BPH1 % Methylated Species % Methylated Species

  4. Figure S4 A DU145 PC-3 * * * % Annexin V positive cells % Annexin V positive cells B DU145 PC-3 % Cells % Cells

  5. Figure S5 B C A ** ** Colonies/well Cells/field Relative growth ** ** ** ** Time (hours) D E Scramble siMMSET pool siMMSET c-ter Cells/field ** **

  6. Figure S6 A Scramble siMMSET pool siMMSET c-ter B C MMSET pool MMSET c-ter Scramble siRNA: * Relative expression Occludin ** ** * ** E-cadherin ** Vimentin Vimentin Fibronectin 1 N-cadherin GAPDH * * ** Relative E-cadherin protein Relative expression γ-catenin E-cadherin Occludin

  7. Figure S7 A RWPE-1 *** Relative expression * ** B DU145 Relative expression * ** ** **

  8. Figure S8 A t(4;14)- t(4;14)+ Relative TWIST1 expression B C p =0.005 400 350 300 250 Relative TWIST1 expression Relative TWIST1 expression 200 150 100 50 0

  9. Figure S9 A Scramble siTwist1 #1 siTwist1 #2 B C D Twist#1 Twist#2 Scr siRNA: Vimentin Cells/field Cells/field ** E-cadherin ** ** ** Occludin GAPDH

  10. Figure S10 A H3K36me2 MMSET ** % Enrichment % Enrichment ** B H3K36me2 MMSET * ** ** % Enrichment % Enrichment * **

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