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Potato Genome Project. Structural Genomics Generation of a comprehensive genomic resource of Solanaceae resistance ( R ) gene sequences of wild potato, tomato and pepper genomes (the SOLAR database) 2.Functional Genomics
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Potato Genome Project Structural Genomics Generation of a comprehensive genomic resource of Solanaceae resistance (R) gene sequences of wild potato, tomato and pepper genomes (the SOLAR database) 2.Functional Genomics Generation of a comprehensive functional genomic resource composed of expression profiles of significant points in growth, development and response to abiotic and biotic stresses, and a set of functionally defined genes identified through virus-induced gene silencing to be involved in host response to pathogens 3.Outreach A training program for high school and college students to teach applications of bioinformatics and data mining for marker development for potato breeding for crop improvement
1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 9 9 10 10 11 11 12 12 1 2 3 4 5 6 7 8 9 10 11 12 Synteny between Solanaceae Genomes • The Solanaceae share highly conserved genomes • Chromosome number and organization • Genome sequence • Sequence and function of gene location and resistance hotspot location Potato Pepper Tomato Resistance hotspots
Potato Tomato GP21 GP179 R1, phyt3, Pi1,Nb, Rx2, Gpa, Grp Bs4 GP179 GP21 Chromosome 5 Resistance Region
S. demissum is an allo-hexaploid Chromosome #5 5A 5B 5C R1, phyt3, Pi1,Nb, Rx2, Gpa, Grp
132D05 GP179 GP21 8~12 BACs per genome ~0.5Mb per genome 132D05 GP179 SPUD237 132D05S GP179 GP21 R1 Sd_Bs4 Sd_Prf R1 A Sd_Prf Haplotype B Sd_Bs4 Sd_Prf C S.demissum Chromosome 5 Resistance Region R1, phyt3, Pi1, Nb, Rx2, Gpa, Grp Chromosome #5
Pepper Tomato phyt3 I I2 L, cmv1, phyt3 S.demissum Chromosome 11 Resistance Region Potato RMcl, Ry, Na, Sen1, R3a, R3b, R5, R6, R7, R8, R9, R10, phyt7
S. demissum is hexaploid Chromosome #11 11A 11B 11C RMcl, Ry, Na, Sen1, N R3a, R3b, R5, R6, R7, R8, R9, R10, phyt7
S.demissum Chromosome 11 Resistance Region RMcl, Ry, Na, Sen1, Chromosome #11 39E18F 56F6R 193I9R A Haplotype B
S.demissum Chromosome 11 Resistance Region R3a, R3b, R5, R6, R7, R8, R9, R10, phyt7 Chromosome #11 381T 373T 51S 11~16 BACs 1.5Mb 381T 373T TG105 381T 373T A Haplotype B C
S.demissum Chromosome 11 Resistance Region R3a, R3b, R5, R6, R7, R8, R9, R10, phyt7 Chromosome #11 381T 373T 51S 12~16 BACs ~1.5Mb 51S 373T GP185 373T 51S A Haplotype B C
Distance tree for all I2/R3 homologues from the MLB locus 6 5 1 100 100 4 100 100 100 100 3 GP185 region GP250 region 0.1 substitution/site 2