160 likes | 275 Views
The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions. TAD. TAD. Sst2 interacts with Ste2 in a DEP-dependent manner in vivo. nucleus. Ub C-terminal hydrolase. NubG. HIS3. TAD. HIS3. TAD. HIS3. HIS3 encodes imidazoleglycerol-phosphate dehydratase
E N D
The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
TAD TAD
Sst2 interacts with Ste2 in a DEP-dependent manner in vivo nucleus Ub C-terminal hydrolase NubG
HIS3 TAD HIS3 TAD HIS3
HIS3 encodes imidazoleglycerol-phosphate dehydratase [D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate hydro-lyase, EC 4.2.1.19] His4A His6 His7 His4B His5 His3 His1 His4C His2 His4C 3-AT H20
imidazole ring triazole ring NH2 CH2—CH—COOH C 3-aminotriazole L-histidine