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Central Dogma （中心法则）

Central Dogma （中心法则）. Reverse Transcription. Adapted from: http://library.thinkquest.org/C0122429/pictures/centraldogma2.gif. Plasmid （质粒）. More Info: http://en.wikipedia.org/wiki/Plasmid. Extra-chromosomal DNA molecule Able to replicate independently of the chromosomal （染色质） DNA

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Central Dogma （中心法则）

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  1. Central Dogma （中心法则） Reverse Transcription Adapted from: http://library.thinkquest.org/C0122429/pictures/centraldogma2.gif

  2. Plasmid（质粒） More Info: http://en.wikipedia.org/wiki/Plasmid Extra-chromosomal DNA molecule Able to replicate independently of the chromosomal（染色质）DNA Usually circular and double-stranded

  3. Plasmid High efficiency of promoter Ori (Origin of Replication) MCS (Multiple Cloning Site) Selective marker

  4. Eukaryotes（真核生物）& Prokaryotes（原核生物） • Chromosome • Nuclear membrane • More complicated to deal with From: http://www.windows.ucar.edu/earth/Life/images/celltypes.gif

  5. Gene regulation (ex. Operon) • RNA polymerase • Promoter • Operator • Repressor • Inducer: ex. IPTG • In an operon, several genes are controlled by one promoter • Example: lac operonin E.coli • Video (http://www.youtube.com/watch?v=oBwtxdI1zvk)

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