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New Flowcell – How Easy?

New Flowcell – How Easy?. New Flowcell – How Easy?. SenCell. SenCell. Small, robust, easy to handle Stepless variable spacing. SenCell - Specifications. Working electrode GC 2mm Reference electrode Salt Bridge - blue ISAAC - green HYREF – black. Sensitivity – wall jet.

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New Flowcell – How Easy?

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  1. New Flowcell – How Easy?

  2. New Flowcell – How Easy?

  3. SenCell

  4. SenCell Small, robust, easy to handle Stepless variable spacing

  5. SenCell - Specifications • Working electrode GC 2mm • Reference electrode • Salt Bridge - blue • ISAAC - green • HYREF – black

  6. Sensitivity – wall jet • Flow cell design • A. thin-layer • B. wall-jet • C. porous flow-through

  7. Cotrell Equation • ECD  Cottrell equation • ilim= 1.47 n F (Fm)1/3 (DA/b)2/3C ilim= C spacer thickness A= WE area A b = spacer thickness C = concentration

  8. Variable spacing – more signal • Stepless variable spacing • Smaller spacing • Higher signal

  9. Spacing

  10. Sensitivity – more signal100um vs 12 um spacing

  11. Sensitivity – more signal • Stepless variable spacing • Smaller spacing • Higher signal 20000 DA 5-HT 15000 S/N of 100 nM standard 10000 5000 0 0 20 40 60 80 100 Spacing SenCell (µm)

  12. [pA] -12 5-HT DA -14 -16 Current -18 100 pM -20 -22 Blank -24 0 1 2 3 4 [min.] Time Sensitivity • LOD • 50 pM DA (0.23 fmol) • 65 pM 5-HT (0.25 fmol) 100 pmole/L standards in Hac + Ringer, 5 uLinjection

  13. Pressure • Higher flow rate due to UHPLC • Smaller spacing down to 10um

  14. Sencell Max Pressure • Leak tight ring • Rating: 25 bar

  15. [nA] 15 5-HT DA 10 Current 5 0 0 0.5 1.0 time (min) UHPLC compatible • High speed analysis • Fast response • Noise level 100 nmole/L standards in Hac, 5 uLinjection

  16. [nA] 15 5-HT DA 10 Current 5 0 0 0.5 1.0 time (min) UHPLC compatible 100 nmole/L standards in Hac, 5 uLinjection

  17. Conclusion • SenCell– The most Easy to use flowcell • Wall jet – performance • Variable spacing - more signal • Less noise by improved fluid dynamics • UHPLC compatible

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