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Learn about the significance of RNA Pol II binding sites, TFIID recognition sites, and DNA modeling, including Watson's contributions to the Double Helix discovery. Explore the importance of promoter and enhancer elements in gene expression.
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What does the word Promoter mean? It is the place at which RNA Pol II binds. But the word is incorrectly used to describe Enhancers plus Promoter.
TFIID recognition site is TATAA How often is this site found in the genome? 1/45 Once every 1000 nucleotides 109 nucleotides or 106 times
Transient transfection More Cells But on a per cell Basis expression levels of -gal is about the same
Watson’s Early attempt To create Double Helix
Jerry Donohue: Worked on dNTPs Told Watson that It was the Keto form Found under physiological conditions