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PCR = DNA ?

PCR = DNA ?. LB 145 January 27, 2011. Does PCR happen inside your body? (How do you raise your temp to 95°C?) PCR was invented in the last 30 years. It exploits our knowledge of another process called ??. Through the process of DNA Replication. Why?.

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PCR = DNA ?

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  1. PCR = DNA ? LB 145 January 27, 2011

  2. Does PCR happen inside your body? (How do you raise your temp to 95°C?) • PCR was invented in the last 30 years. It exploits our knowledge of another process called ??...

  3. Through the process of DNA Replication

  4. Why? • Each cell must have a way to copy its entire genome • Cell Division • Damage Control Where does this take place?

  5. Check this out • http://www.youtube.com/watch?v=teV62zrm2P0&feature=related • http://www.youtube.com/watch?v=AGUuX4PGlCc

  6. PCR vs. DNA Replication • Similarities? • Copy DNA • Move 5’-3’ via a polymerase enzyme • require primers

  7. PCR vs. DNA Replication • Differences? • Constant temperature (body temp is 37C) • Enzymes are the workhorses • All 3 stages happen simultaneously • Multiple enzymes vs. Taq Polymerase • Leading/Lagging Strand • RNA primers vs DNA primers

  8. So, what are we doing again? • PCR is selective DNA replication, it exploits our knowledge of the DNA rep. • Instead of using difficult (and expensive) enzymes in the body we used more controllable, predictable PCR machines.

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