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Elias Greenbaum, Ph.D.

Elias Greenbaum, Ph.D. Corporate Fellow Oak Ridge National Laboratory. Nanoscale Photovoltaics & Artificial Sight. Elias Greenbaum Oak Ridge National Laboratory Oak Ridge, Tennessee. February 24-27, 2009 Orford, Quebec, Canada. Acknowledgements. Mark S. Humayun Christian P. Pannisi

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Elias Greenbaum, Ph.D.

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  1. Elias Greenbaum, Ph.D. Corporate Fellow Oak Ridge National Laboratory

  2. Nanoscale Photovoltaics & Artificial Sight Elias Greenbaum Oak Ridge National Laboratory Oak Ridge, Tennessee February 24-27, 2009 Orford, Quebec, Canada Presentation_name

  3. Acknowledgements Mark S. Humayun Christian P. Pannisi Ken Yoshida Barbara R. Evans Hugh M. O’Neill James W. Lee Ida Lee Tanya Kuritz Charlene Sanders Miguel Rodriguez, Jr. Barry Bruce Jennifer Millsaps DOE Office of Basic Energy Sciences, Office of Biological and Environmental Research Presentation_name

  4. Photosystem I Platinized Photosystem I Experiment H+ H2 H+ H+ [PtCl6]2– + 4e– Pt + 6Cl – Platinum Ascorbic Acid Plastocyanin e- Light e-

  5. Differences in H2 Yield With Decreasing Chl Concentration 0.012 0.010 360 mg/mL Chl / h / mgChl 0.008 0.006 IntegratedYield: 0.004 µmol H 2 0.002 0.025 mmol H2 / mg Chl 0.000 0.20 h / mgChl 24.5 25.0 25.5 26.0 26.5 27.0 100 mg/mL Chl 0.15 Time(h) 0.10 Integrated Yield: µmol H2 / 0.05 0.53 mmol H2 / mg Chl 0.00 21.5 22.0 22.5 23.0 23.5 24.0 24.5 Time(h) 2 / h / mgChl 50 mg/mL Chl 1 µmol H2 Integrated Yield: 3.94 mmol H2 / mg Chl 0 21.5 22.0 22.5 23.0 23.5 24.0 Time(h) Light off Light on Light off Light on Light off Light on

  6. PSI PSI H H H H H H H H H H H O O O O O O O O O O O S S S S S S S S S S S Au PSI on a Heterostructure

  7. d c h Light 0 V – – – + + + b a 0 nm Au Measurement of Electrostatic Potential in Nanometer-Resolution: Schematic Illustration Kelvin Probe Method in Lift Mode

  8. 30 nm 5 V a b Light on Light on 0 V 15 nm -5 V 0 nm Light Off Light Off AFM and KFM Images of PS I Reaction Centers Immobilized on Gold

  9. PS I Photovoltage Topography: Summary of Three Types of Measurements The vertical arrows in above figure indicate three selected voltage points we used in this table: the two extremities and the center.

  10. Conceptual Illustration of Idea

  11. Add (proteo) liposomes Load with the fura-3 dye WERI-RbI retinoblastoma cells fused with (proteo)liposomes Illuminate with 600-700 nm WERI-RbI retinoblastoma cells fused with (proteo)liposomes and loaded with fura-3 WERI-RbI retinoblastoma cells

  12. Spectral Properties of System

  13. RECONSTITUTION FIRST STEP: PSI RECONSTITUTION INTO PROTEOLIPOSOMES

  14. INCORPORATION NEXT STEP:PSI INCORPORATION INTO MAMMALIAN CELLS

  15. Light triggers increase in cytosolic Ca2+ in proteoliposome-treated cells

  16. Kinetic Time Course Experiments in the Presence and Absence of PSI and External Calcium

  17. Spatial Distribution of the Electric Potential From Photosystem I Reaction Centers in Lipid Vesicles

  18. Distribution of the Electric Potential Close to a PSI Reaction Center Inserted in the Vesicle Membrane The Role of Ionic Strength 0 0.1 M IEEE Transactions on Nanobioscience, Vol. 7, No. 2, June 2008, Pennisi, Greenbaum & Yoshida

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