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Connective Tissue Identification and Analysis Guide

Learn to identify different connective tissue cells, protein fibres, and types to gain insight into tissue properties. Includes practical observations and microscopy tasks. Understand unique features of various connective tissues.

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Connective Tissue Identification and Analysis Guide

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  1. 003 Connective Tissue Objectives: The objective of this class is 1. To identify different types of connective tissue cells, protein fibres and connective tissue types that provide unique properties to different tissue types. Please indicate the magnification of drawings by stating the objective lens used. 1 LOOSE OR AREOLAR CONNECTIVE TISSUE – SLIDE 118 – SECTIONED MATERIAL SLIDE 58 – SPREAD PREPARATION Try to identify the following cell types: FIBROBLASTS, ADIPOSE CELLS, MAST CELLS, MACROPHAGES, and if possible RED & WHITE BLOOD CELLS. (MACROPHAGES LABELLED WITH CARBON PARTICLES CAN BE SEEN ON SLIDE 144). We will demonstrate the MAST cells labelled with Toluidine Blue on the TV monitors. Look at the spread preparation on slide 58; You should see two types of protein fibre. Q 1. Which of these is thinner and branched compared with the thicker wavy fibres? Q 2. What holds these protein fibres and cells together in this preparation? Q 3. How did you distinguish between the blood capillaries and protein fibres? 118 58

  2. 2 SUBCUTANEOUS TISSUE – SLIDE 116 There is an abundance of protein fibres bundles staining deep blue in this section. This section also contains large clusters of purple staining lymphoid tissue. Make a simple drawing to highlight the main features. Q 4. What protein fibres stain deep blue in this section? Q 5. What are the red cells? DENSE REGULAR CONNECTIVE TISSUE – SLIDE 121 This section contains both muscle and tendon. Observe the tendon cells insinuated between the protein fibres. 3 Q 6. What colour are the muscle cells? Q 7. What is the most abundant constituent of the tendon? Q 8. What is the orientation of protein fibres in the tendon?

  3. DENSE IRREGULAR CONNECTIVE TISSUE – SLIDE 78 …dermis of skin Q 9. Why is this connective tissue described as irregular? Q 10. What are the pink stained dense interwoven bundles of protein fibres composed of? Q 11. What is the purple staining major cell type found beneath the surface epidermis? 4 ELASTIC CONNECTIVE TISSUE – SLIDE 122 LIGAMENTUM NUCHAE Most of the protein fibres are stained yellow in this section. Q 12 . What are these protein fibres composed of? Note fibroblasts are stained black. 5 ADIPOSE TISSUE – SLIDE 118 for UNILOCULAR WHITE. 6 Q 13. Where is the nucleus located in these cells? Q 14. What is the major component of these cells and why do they appear empty in this section? SLIDE 86 for MULTILOCULAR BROWN. Q 15. Why is this tissue called multilocular? Q 16. Are all the adipocytes in this sample multilocular or do you find interspersed any unilocular cells?

  4. RETICULAR CONNECTIVE TISSUE FIBRES • - SLIDE 21 LIVER or • SLIDE 125 LYMPH NODE • Examine one of these sections and note the loose interwoven network created by the • reticular fibres that are stained black. • Q 17. Why is this protein fibre most appropriate in these tissues? • Q 18. What is it composed of and how does it compare with other protein fibre types? 7

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