Biomoles RedTrack & GreenTrack Gels Comparisons

1. Biomoles RedTrack & GreenTrack Gels Comparisons Jan 2013 Biomoles Inc. Tel: 206-877-0969 biomole@biomoles.com www.biomoles.com

2. Key Specification Features of TheRedTrack & GreenTrack Precast Gels Biomoles’ new generationRedTrack&GreenTrackPrecast Gelswhich are away from ethidium bromide, not only address health and safety concerns, but provide superior performance & competitive pricing Non-hazardous, non-toxic, safe to both researchers and environmental health. Gels can be disposed in garbage cans even after dried up. High sensitive, sharp & clear DNA bands, remarkable clean background which enables DNA & RNA quality separation, as well as mini-fragments, tiny amount analysis (as low as 0.5ug). Separateand visualize impurity contaminants from DNA & RNA. Long term (2 days under RT, and 10mon at 4oC) fresh DNA bands gel preservation which are not only great convenience for delayed gel imaging after electrophoresis, but enables long-term DNA gel excise, mobile observation and even overtime presentation.

3. Biomoles RedTrack & GreenTrack Precast Gels ---------------------------------- 8/16 well & 14 wells fit all gel box formats

4. Biomoles Customized Multi-Peppit 32-Well Gels ---------------------------------- 32 well multi-peppit gel fits 15cm of gel box, and directly for 48/96 DNA samples

5. Biomoles RedTrack & GreenTrack Precast Gels ----------------------------------

6. Biomoles RedTrack & GreenTrack Precast Gels ----------------------------------

7. Competitive Advantage in Precast Gels RedTrack &GreenTrackGels Compared with the ethidium bromide gel: • Superior sensitivity • Better performance • Easy-to-handle • Very clean background • Able to detect smallest size and tiny amounts of DNA with high resolution • Safe, non-toxic to replace traditional mutagen • Competitive Pricing RedTrack/GreenTrack EtBr 50bp DNA ladder, 4.5ng per lane RedTrack GreenTrack EtBr10bp – 4kb, 5ng per lane

8. Innovative Tools and Diagnostics ---------------------------------- Precast Agarose Gel Sensitivity GreenTrack & RedTrack RedTrack 50bp DNA ladder, 4.5ng per lane 10bp – 4kb, 5ng per lane

10. Innovative Tools and Diagnostics ---------------------------------- 1st RNA Report from A Researcher Note: the visibility of RNA bands could be improved by loading the recommended concentration of Red/Orange Loading dye RNA as low as 0.5ng per well Under insufficient Red/Orange Loading Dye 1.0% RedTrack Precast Gel

11. Extending DNA Gel-Life Fresh image Red/&Green TrackPrecast Gels preserve DNA samples for more than10 Monthsvs the current 15 minuntes --- A Historical Solution! RedTrack Gel Fresh Image taken in Aug 16, 2007. Plasmid DNA Isolated from Biomolès Kit This feature is also a great advantage for delayed DNA gel imaging after electrophoresis, DNA gel excise, and even overtime presentation or mobile observations of the DNA bands 10 months later RedTrackGel Image 10 month Later, taken at May 8th, 2008. Plasmid DNA Isolated from Biomolès Kit

12. Innovative Tools and Diagnostics ---------------------------------- Background and DNA Band Comparison Between the Ethidium Bromide Based Gels & The RedTrack Precast Gels Ethidium Bromide Gel RedTrack Agarose Gel --- 20bp DNA with or without showing the bands

13. Innovative Tools and Diagnostics ---------------------------------- DNA bands can be excised directly from the gel without hinder block as other precast gels may have

14. How To Use Biomoles Precast Gels A. Open The Gel Pouch Using Scissors

15. How To Use Biomoles Precast Gels B. Pill Off The Seal Cover, Pour Out Completely The Residual Buffer

16. How To Use Biomoles Precast Gels C. Remove The Up & Down Sides of the Tray (with gel in) with Ladder Banks By Flexing Them Down & Away One Side at a time (indication images)

17. How To Use Biomoles Precast Gels D. Measure the internal height of your gel box to make the 14well Tray Holder fit to it. Insert the side of the tray into the tray holder clip, placing the gel tray at the bottom of the gel box, hang another end of the tray holder over the gel box wall.

18. How To Use Biomoles Precast Gels E. If you have a sample gel and do not have the gel tray holder, simply place The Tray at The Bottom of the Gel Box With Left Side Flush With The Side Wall of The gel box. Use peppit tip to stabilize the gel trays.

19. How To Use Biomoles Precast Gels F. Add Buffer (1XTAE or TBE) into the gel box. Load DNA or RNA samples. Cover the gel box, run Electrophoresis

20. How To Use Biomoles Precast Gels F. Biomoles 8-well & 16-well of Precast Gels Fit The Standard 7cm Width Of Gel Box As The Bio-Rad’s Mini-Sub Cell GT

21. Safe Disposal Biomoles Precast Gels You can dispose the precast gel after using into the regular garbage can let it dry out, it will be totally safe and non-hazardous. Suggestion: please advise those still using ethidium bromide based gels to eliminate the mutagenic toxicity to human health. Thanks for your support. Contact: biomole@biomoles.com, or 206-877-0969

22. Biomoles DNA Purification Kits--- DNA PurityComparison - Isolated genome DNA from tissue & buffy coat samples Compared with Q & M kits DNA purity 15-20% greater DNA yields 40% higher greater Transfection grade Plasmid DNA Use for any downstream applications without further process

23. Thank You