Di Xu 1 , Giulianna Borges 2 , Baoli Yang 3 and Curt D. Sigmund 2,4

1. P=0.036 Conclusions Introduction (http://mednote.dk/images/Fysiologi_paulev_06-4.jpg) +/+ N=4, -/- N=4 +/+ N=4, -/- N=4 +/+ N=4, -/- N=3 P=0.024 * * * Hypothesis +/+ N=4, -/- N=4 P<0.001 +/+ N=4, -/- N=4 P=0.035 * * Methods * * Genetic Disruption of Secreted Renin Preserves Expression of mRNA Encoding Intracellular Renin in the Brain Di Xu1, Giulianna Borges2, Baoli Yang3 and Curt D. Sigmund2,4 1Interdisciplinary Genetics Program, 2Department of Internal Medicine, 3Department of Ob/Gyn, and 4Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa Results Ablation of sRen Leads to Hypotension, Decreased Heart Rate and Blunted Baroreflex Activity Preservation of icRen Does Not Rescue the Lethality Caused by Loss of sRen Renin Isoforms play differential roles in cardiovascular regulation and fluid homeostasis in the brain and kidney. Ablation of sRen Leads to a Trend of Increased Fluid Intake /Urinary Output and Decreased Body Weight/ Brown Adipose Lethality of sRen KOs is Related to Renal Atrophy Generation of Conditional Knockout Mice WT • Secreted renin is essential for appropriate renal development and survival. • Preservation of intracellular renin cannot compensate for loss of secreted renin. • Disruption of secreted renin along is sufficient to cause hypotension. • Crippling of secreted renin leads to decreased body weight. KO