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Evaluation of Plant Growth Promoting Characteristics of Arsenic-resistant Bacteria

Evaluation of Plant Growth Promoting Characteristics of Arsenic-resistant Bacteria. Yupa Chromkaew and Arawan Shutsrirung Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Thailand . Arsenic.

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Evaluation of Plant Growth Promoting Characteristics of Arsenic-resistant Bacteria

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  1. Evaluation of Plant Growth Promoting Characteristics of Arsenic-resistant Bacteria YupaChromkaew and ArawanShutsrirung Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Thailand

  2. Arsenic Arsenic, a metalloid widely distributed in soil, waters, air and all living matter, released both from natural and anthropogenic sources, from the weathering of rocks or by mining industries and agricultural practices

  3. Arsenic • high arsenic levels in many cultivated fields on highland areas of northern Thailand, which are mainly because of high input of agro-chemicals for crop production (Shutsrirung, 2012). • Arsenic contamination to soil and water resources on highland areas poses an environmental and human health problem

  4. Arsenic • Arsenic is very toxic to plant and microorganism. • Plants can uptake and accumulate arsenic in their tissues. • Thus, a human health risk concern arises regarding accumulation of As in plant grown in these soils.

  5. Microbial • Microbial populations are known to affect heavy metals mobility and availability to the plant through release of chelating agents, phosphate solubilization, synthesizing phytohormone, fixing nitrogen, producing siderophore and other nutrients. • Therefore, the potential and the exact mechanism of bacteria to detoxify heavy metals have recently received more attention.

  6. MATERRIALS AND METHODS • In our previous study, bacterial isolates were screened for their ability in arsenic detoxification. • The arsenic-resistant isolates could oxidize highly toxic form of arsenic, arsenite, into less toxic form of arsenate. • Five isolates (7, 8, 11, 19, 29) were selected from the previous experiment to evaluate their ability in IAA, siderophore production, phosphate solubilization and germination enhancement of Chinese Kale seed.

  7. MATERRIALS AND METHODS

  8. Indole-3-acetic acid (IAA) productiondescribed by Gordon and Weber (1951) until reach maximum growth for 5 days

  9. Phosphates solubilization(Gaur, 1990) for 5 days

  10. Siderophore production(Schwyn and Neilans, 1997) until reach maximum growth for 14 days

  11. Seed germination test incubator for 3 days

  12. RESULTS ANDDISCUSSION

  13. Siderophore production by arsenic-resistant bacteria

  14. Effects of arsenic-resistant isolates on seed germination and root length of Chinese Kale control Isolate 7

  15. CONCLUSION

  16. All the five arsenic-resistant isolates performed high ability to produce of IAA (9.06-20.32 mg/L) at only 1 mg/ml of tryptophan. • In our experiment, all isolates were able to solubilize P and isolate 7 gave the highest amount of soluble P in Pikovskaya’s medium and Pikovskaya’s medium supplemented with sodium arsenite and aluminum (55.56 and 100.04 mg/L).

  17. Only isolate 11 was able to produce siderophore. Siderophore can enhance availbility of Fe3+ • Isolate 29 showed the best performance to increase the seed germination and isolate 7 produced the highest root length. • Our results suggested that IAA producing isolates showed a tendency to enhance seed germination and root length of Chinese Kale however we suggest that, to obtain more obvious growth-promoting activity of inoculated isolates.

  18. Using these bacterial isolates could be a realistic and desirable strategy for maintaining crop production in arsenic contaminated soils as well as reduction of arsenic uptake by crops. • The ability of tested bacterial isolates to produce plant growth-promoting compounds and stimulatory efficiency on seed germination showed their potential to be utilized as bio-inoculant for better crop production in arsenic-contaminated agricultural soils.

  19. Nevertheless, additional research will be necessary to identify effects of these arsenic-tolerant and plant growth-promoting bacterial isolates on detoxification of soil arsenic and enhancement of plant growth and yield under controlled and field conditions. http://victoria-gardens.net/shop.php?id=3

  20. ACKNOWLEDGEMENT • Highland Research and Development Institute (Public Organization)

  21. Thank You for your attention

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