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x Blast y pour tous

x Blast y pour tous. Recherche BLAST 1,2,3,4,5. Choisir sa séquence Choisir le programme BLAST Choisir la banque Choisir les paramètres optionnels … et attendre un peu. séquence. Domaines conservés. Banque non redondante. GO.

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x Blast y pour tous

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  1. xBlasty pour tous

  2. Recherche BLAST 1,2,3,4,5 • Choisir sa séquence • Choisir le programme BLAST • Choisir la banque • Choisir les paramètres optionnels … et attendre un peu

  3. séquence Domaines conservés Banque non redondante GO

  4. Séquence AC, FASTA ou text

  5. Choisir un programme BLAST

  6. ProgrammeEntréeBanque 1 blastn DNA DNA 1 blastp protéine protéines 6 blastx DNA protéines 6 tblastn protéine DNA 36 tblastx DNA DNA

  7. … un ADN peut, potentiellement, coder 6 protéines 5’ CAT CAA 5’ ATC AAC 5’ TCA ACT 5’ CATCAACTACAACTCCAAAGACACCCTTACACATCAACAAACCTACCCAC 3’ 3’ GTAGTTGATGTTGAGGTTTCTGTGGGAATGTGTAGTTGTTTGGATGGGTG 5’ 5’ GTG GGT 5’ TGG GTA 5’ GGG TAG

  8. Choix de la banque nr = non-redundant (most general database) dbest = database of expressed sequence tags dbsts = database of sequence tag sites gss = genomic survey sequences htgs = high throughput genomic sequence

  9. OPTIONS Chercher des domaines conservés

  10. OPTIONS Entrez! Filter Expect Word size organisme Scoring matrix

  11. OPTIONS RBP4 vs RBP4 avec option FILTRE

  12. Filtre ON

  13. Filtre OFF

  14. program query database taxonomy

  15. taxonomy

  16. Cut-off: .05? 10-10?

  17. Alignment view

  18. Alignment view

  19. MEUH !

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