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Bactiquant Water Rapid Onsite Screening for Assessing Legionella Risk

Bactiquant Water Rapid Onsite Screening for Assessing Legionella Risk. Presented by Lisa Rogers.

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Bactiquant Water Rapid Onsite Screening for Assessing Legionella Risk

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  1. Bactiquant Water Rapid Onsite Screening for Assessing Legionella Risk Presented by Lisa Rogers

  2. “Legionellosis emerged because of human alteration of the environment, since Legionella species are found in aquatic environments, and thrive in warm water and warm, damp places, such as cooling towers” Legionella and the prevention of legionellosis, WHO 2007

  3. World Wide • Since 1976, 35 outbreaks reported 2-800 cases • 11 in NA (US/CA) • 14 in AUS/NZ • 10 in Europe • Sources • 13 Cooling towers • 5 each, Spas/hot tub, DHW, AC, and 4 unknowns • Misc include potting mix, fountain, industrial systems

  4. Concerns • CDC estimates 8,000 to 18,000 people are hospitalized each year in the U.S. Mortality rate is approximately 15% • Legionella bacteria are found naturally in the environment • French study found thatL. pneumophila can travel airborne at least 6 km from its source • Norway study found high velocity, large drift & high humidity in air scrubber… spread probably for >10 km • WHO estimates the cost savings to implement preventive plans is $1-3M USD per life saved. (1200 x 1M= $1.2B)

  5. Ideal Water Conditions • Temperatures between 20° and 50°C (68° - 122°F) • Optimal growth range is stagnant water 35° - 46°C [95° - 115°F] • pH between 5.0 and 8.5 • Available iron and copper • Sediment/scaling promote growth of commensalmicroflora • Other micro-organisms including algae, flavobacteria, and Pseudomonas, which supply essential nutrients for growth of Legionella or harbor the organism (amoebae, protozoa) • Low biocide concentrations

  6. Water Sampling Guidelines Table III:7-1. COLONY FORMING UNITS (CFU) OF LEGIONELLA PER MILLILITER Action 1: Prompt cleaning and/or biocide treatment of the system. Action 2: Immediate cleaning and/or biocide treatment. Take prompt steps to prevent employee exposure. Source : OSHA, 1999 CDC recently testified that there is no safe level !

  7. Current Sampling Strategy Culture Plate Analysis • 3-5 days for HPC • 7-10 days for Legionella • Planktonic, highly variable, overloading, background interferences PCR • fast but expensive

  8. How BQW can help Rapid test can be performed in 15-30 mins Easy Sampling and Analysis EPA ETV verified methodology

  9. Enzyme substrate Fluorescent compound released + Bacteria-enzyme Principle Method

  10. Why is Bactiquant Technology interesting ? • Culture-independent technique • Quantification of a naturally occuring hydrolase enzyme activity present in bacteria • High specificity to bacteria, little background interference • Use of fluorescence technology = high sensitivity • Concentration Step = increased sensitivity and significant reduction in detection time

  11. Laboratory Comparison BQW

  12. EPA ETV Report Excerpt Table 2: Linearity: BQ Value vs Concentration SourceBattelle / US EPA -2011

  13. EPA ETV Study Excerpt High reproducibility SourceBattelle / US EPA -2011

  14. Table 3. Bactiquant®-test Repeatability and Inter-Assay Reproducibility EPA ETV Report Excerpt Table 3 summarizes the repeatability and inter-assay reproducibility results for Bactiquant®-test using two bacterial cultures in water. Two different people analyzed 4 samples of each culture, using different fluorometers.

  15. Case Studies

  16. Critical care facility, Courtesy of Clinical Microbiology Institute, Viborg Hospital, Denmark 2006. BQ value 1000 BQ value 100 X-axis Sample No. (1-70) Y-axis Log10(adjusted fluorescence) and Log10(cfu) Legionella pneumophila serotype 2-14 BQ value 10

  17. Australia Case Study • Wesley Hospital, Queensland: 2 cases, 1 death • 5 Building, 560 bed facility • Heat Treatment for 2 weeks @ 70C ( 158F) • Over 3000 samples collected BQ, Legionella, HPC • 13 days to reopen, 29 days fully operational • BQ used to screen, verify cleaning efficacy and validate post treatment

  18. Excerpt from: The Australian Hospital Engineer, September 2013 “CETEC made use of the Bactiquant® water technology for the rapid screening of total bacterial loading within the potable water network. This technology allowed CETEC in under an hour to obtain results superior to plate count for the network. The ability to obtain rapid results as compared to 4 to 10 day timeframe for conventional methods presented significant time, logistical and financial savings.”

  19. New Studies The Danish Health Institute has started a two year project now with multiple sites looking at pre-screening as well as post treatment efficacy. Hopefully, some results from that study will be forthcoming later this year.

  20. Pros and Cons • Rapid onsite testing aids in identifying hotspots • Validate the efficacy of cleaning methods • Monitor for changes in system quickly • HPC nor BQW a perfect predictor of Legionella • Not a substitute for direct Legionella analysis

  21. Do you have any questions? Thank you for listening!

  22. Lisa Rogers Lrogers@mycometer.com 813-831-6511 www.mycometer.com Booth on Aisle 3000

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