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The Hammerling Experiment

The Hammerling Experiment. By: Danny Nemeth Michael Leonard Allie Wrabel. Acetabularia . Danish biologist Joachim Hammerling needed cells large enough to operate on conveniently and differentiated enough to distinguish the pieces.

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The Hammerling Experiment

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  1. The Hammerling Experiment By: Danny Nemeth Michael Leonard Allie Wrabel

  2. Acetabularia • Danish biologist Joachim Hammerling needed cells large enough to operate on conveniently and differentiated enough to distinguish the pieces. • He chose the unicellular green alga Acetabularia, which grows up to 5 centimeters, as a model organism for his investigations.  • Just as Mendel used pea plants and Sturtevant used fruit flies, Hammerling picked a model organism that was suited to the specific environmental question he wanted to answer, assuming that what he learned could then be applied to other organisms. 

  3. Acetabularia • Individuals of the genus Acetabularia have distinct foot, stalk, and cap regions; all are differential parts of a single cell.  The nucleus is located in the foot. • As a preliminary experiment, Hammerling amputated the caps of some cells and the feet of others. • He found that when he amputated the cap, a new cap regenerated from the remaining portions of the cell (foot and stalk).  • However, when he amputated the foot, no new foot regenerated from the cap and stalk. • Therefore, Hammerling hypothesized that the hereditary information resided within the foot of Acetabularia. 

  4. Surgery on Single Cells • Testing the Hypothesis: • select individuals from two species of the genus Acetabularia • Caps of cells look different • A. mediterranea • Disk shaped cap • A. crenulata • branched, flowerlike cap • 1. Cut the cell into 3 parts (Cap, Stalk, Foot) • 2. Combine the foot and cap of the cell with the stalk of the different cell • 3. Watch how the cap grows on both cells.

  5. Conclusion The Experiment determined that: • If the caps would grow the same as before,  the heredity information would have to be stored in the foot of the cell. • If the cell would have regrown different caps,the nucleus would not be found in the foot.  • Now we understand that genetic instructions pass from the nucleus in the foot upward through the stalk to the developing cap.

  6. Thank You For Your Time :)

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