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Supplemental figure. A. Wt b 3-2 b 3-3 b 3-4 b 3-5 b 3-6 mock mock mock mock. B. NP-9 3 -3. NP-9 3 -4. NP-9 3 -5. NP-9 3 -6. v 3 (FITC) and F-actin (TR). Supplemental figure. NP-9 β 3 clones: expression of β 3 and cell morphology.
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Supplemental figure A Wt b3-2 b3-3 b3-4 b3-5 b3-6 mock mock mock mock B NP-93-3 NP-93-4 NP-93-5 NP-93-6 v3 (FITC) andF-actin (TR) Supplemental figure. NP-9β3 clones: expression of β3 and cell morphology. A. Different clones for NP-9β3 transfected cells were grown in complete medium. After 24 hours, 40 g of total protein extracts were immunoblotted using antibody against β3: β3(AP3). wt: parental, mock: mock-transfected and β3: β3-transfected. B. Cells were seeded on glass coverslips, fixed, permeabilized and stained for: αvβ3 integrin (FITC) and F-actin (TR). All samples were analyzed using confocal microscopy. Bars, 20 m. 45