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Presented to: NPS Project Review Meeting Wednesday, December 12, 2012 LDEQ Baton Rouge, LA

The Mississippi River Basin Initiative (MRBI) Watershed Water Quality Monitoring of Bayou Chene an Lacassine Bayou. Presented to: NPS Project Review Meeting Wednesday, December 12, 2012 LDEQ Baton Rouge, LA Presented by: Durga D Poudel UL Lafayette. Introduction Objectives

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Presented to: NPS Project Review Meeting Wednesday, December 12, 2012 LDEQ Baton Rouge, LA

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  1. The Mississippi River Basin Initiative (MRBI) Watershed Water Quality Monitoring of Bayou Chene an Lacassine Bayou Presented to: NPS Project Review Meeting Wednesday, December 12, 2012 LDEQ Baton Rouge, LA Presented by: Durga D Poudel UL Lafayette

  2. Introduction • Objectives • Materials and Methods • Results and Discussions • Summary • Acknowledgements

  3. Introduction

  4. Materials and Methods Weekly field measurements and sampling: Water depth, DO, temp. pH, turb., cond. Laboratory determination: TSS, TDS, TS, Nitrite, Nitrate, TKN, TP, SRP, Cl, Fl, SO4, BOD5

  5. Fish and macroinvertibrates sampling

  6. For each castnet (12ft radius cast net with 3/8th inch mesh size) sample, we combined the fish from 5 cast net throws.  • For each dipnet sample, we sample 5 minutes.  • We do this 4 times per site (resulting in the 4 replicates) for a total of 20 cast net throws and 20 minutes of dipnetting per site.  • Collecting the fish and benthic samples takes 2.5 to 3 hours per site, for a crew of three people.  • Benthic samples were taken with a petite Ponar grab, and sieved in the field (500 micron screen). Sample sorting/identification is ongoing.

  7. Petite Ponar Grab sampler > 500 µm fraction to be preserved in 10% formalin with Rose Bengal stain.

  8. Results and Discussion

  9. Fish abundance measures, by site, bayou and overall. Shown are mean numbers per cast throw (5 throws/replicate) and numbers per minute of collection by dipnet (5 min. per replicate) for the four replicates at each site combined. * Mosquitofish (Gambusia affinis) excluded due to sometimes extreme numerical dominance (up to 99% of total number of individuals)

  10. Fish species collected

  11. Field observations

  12. Summary • Weekly data collection helps in understanding water quality variability and watershed conditions better. • Monthly flow data for sampling locations are being collected with the help of LDEQ which will allow us in calculating loading rates for the 9 Zones per unit area and determining the effectiveness of BMPs in reducing nutrients, sediments, and biological oxygen demanding substances entering the bayous. • Biological data on species diversity and abundance will be useful in assessing the effectiveness of BMPs on improving watershed health and aquatic life.

  13. Acknowledgements We like to acknowledge LDEQ and EPA Region 6 for funding this project. We also like to thank NRCS, LDAF, LDEQ staff and other stakeholders for helping us in this project

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