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An Affordable Evaporative Light Scattering Detector for Every HPLC 蒸发光散射检测器

An Affordable Evaporative Light Scattering Detector for Every HPLC 蒸发光散射检测器. Melissa J. Wilcox Alltech Associates, Inc. 2051 Waukegan Road • Deerfield, IL 60015 Phone: 1-800-ALLTECH • Web Site: www.alltechWEB.com. PP083. The New ELSD 800 – The First High-Performance, Low-Cost ELSD.

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An Affordable Evaporative Light Scattering Detector for Every HPLC 蒸发光散射检测器

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  1. An Affordable Evaporative Light Scattering Detector for Every HPLC蒸发光散射检测器 Melissa J. Wilcox Alltech Associates, Inc. 2051 Waukegan Road • Deerfield, IL 60015 Phone: 1-800-ALLTECH • Web Site: www.alltechWEB.com PP083

  2. The New ELSD 800 – The First High-Performance, Low-Cost ELSD

  3. Small and Stackable

  4. Unique Method of Detection • Three steps: •  Nebulization •  Mobile Phase Evaporation •  Detection

  5. Step 1: Nebulization • Column effluent passes through nebulizer needle •  Mixes with nitrogen gas •  Forms dispersion of droplets

  6. Step 2: Mobile Phase Evaporation Droplets pass through a heated zone  Mobile phase evaporates from the sample particle Dried sample particles remain

  7. Step 3: Detection Sample particles pass through an optical cell Sample particles interrupt laser beam and scatter light Photodiode detects the scattered light

  8. The ELSD Improves Baseline Stability and Detection Sensitivity Compared to RI 10278 1. Fructose 2. Glucose 3. Sucrose 10277 Column: Prevail™ Carbohydrate ES, 5µm, 53 x 7mm (Part No. 35104) Mobile Phase: Acetonitrile:Water (75:25) Flowrate: 2.0mL/min RI ELSD

  9. The ELSD’s Response Accurately Reflects Equal Concentrations of Two Brominated Flame Retardants 10260 10261 1. Hexabromocyclododecane (500ppm) 2. Tetrabromobisphenol A (allyl ether) (500ppm) Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056) Mobile Phase: Acetonitrile:Water (95:5) Flowrate: 1.0mL/min UV at 254nm ELSD

  10. The ELSD Responds to All Components in this Antihistamine Formulation 10274 1. Diphenhydramine 2. PEG, Gelatin 3. Sorbitan 4. Glycerol 5. Sorbitol 10275 Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101) Mobile Phase: Acetonitrile:0.1% Ammonium Carbonate, pH 6.5 (80:20) Flowrate: 1.0mL/min UV ELSD

  11. Using Acetone in a Mobile Phase Gradient Results in a 50% Reduction in Analysis Time Unmodified Mobile Phase Modified Mobile Phase 1.  Fructose 2.  Glucose 3.  Maltose 4.  Maltotriose 5.  Maltopentaose 6.  Maltohexaose 7.  Maltoheptaose 9350 9360 Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: ACN B: Water Gradient:(Time,%B),(0,20),(50,35) Flowrate: 1.0mL/min Detector: Alltech ELSD Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: ACN:Acetone(75:25) B: Water Gradient:(Time,%B),(0,24),(18,50) Flowrate: 1.0mL/min Detector: Alltech ELSD

  12. Detect Difficult Samples like Triglycerides without Derivatizing 1. LLO 2. LLP 3. OOL 4. POL 5. PPL 6. OOO 7. OOP 8. PPO 9. OOS 9515 Column:Alltima™ C18, 3µm, 150 x 4.6mm Mobile Phase: A: Dichloromethane B: Acetonitrile Gradient:(Time,%B),(0,70),(10,55), (18,70),(20,70) Flowrate: 1.5mL/min Detector: Alltech ELSD

  13. Use ELSD in Parallel with MS to Obtain Maximum Structural and Concentration Information 9209 1. FMOC-Ala-OH 2. FMOC-Trp-OH 3. FMOC-Arg(Pmc)-OH 9208 Column: YMC ODS-A, 5µm, 50 x 4.6mm Mobile Phase: A: 0.05% TFA in Water B: 0.035% TFA in Acetonitrile Gradient:(Time,%B),(0,30),(3.7,95),(4.2,95) Flowrate: 3.5mL/min MS ELSD

  14. Replace Fluorescence Detectors with ELSD to Simply Methods by Eliminating Pre- or Post-column Derivatization 1.  Glycine (Gly) 2.  Serine / Asparagine (Ser/Asn) 3.  Aspartic Acid (Asp) 4.  Glutamine (Gln) 5.  Alanine / Threonine (Ala/Thr) 6.  Glutamic Acid (Glu) 7.  Cysteine / Lysine (Cys/Lys) 8.  Histidine (His) 9.  Proline (Pro) 10.  Arginine (Arg) 11.  Valine (Val) 12.  Methionine (Met) 13.  Tyrosine (Tyr) 14.  Isoleucine (Ile) 15.  Leucine (Leu) 16.  Phenylalanine (Phe) 17.  Tryptophan (Trp) 9413 Column: Prevail™ C18, 5µm, 250 x 4.6mm (Part No. 99210) Mobile Phase: A:5mM Heptafluorobutyric Acid, pH 1.0 w/0.7% TFA B:Acetonitrile Gradient:(Time,%B),(0,0),(6,0),(8,15),(25,35) Flowrate: 1.0mL/min Detector: Alltech ELSD

  15. Fat Soluble Vitamins — Micro Scale 1. Vitamin A (100ng on-column) 2. Vitamin E (160ng on-column) 3. Vitamin K1 (130ng on-column) 10279 Column: Alltima™ C18, 3µm, 150 x 1mm (Part No. 88384) Mobile Phase: Methanol:Chloroform (90:10) Flowrate: 50µL/min Detector: Alltech ELSD 800

  16. Catechins in Green Tea — Prep Scale 10140 1. Caffeine2. Epicatechin3. Epigallocatechin Gallate Column: Alltima™ C18, 5µm, 150 x 22mm (Part No. 81106) Mobile Phase: (87:13) 0.05% TFA:Acetonitrile Flowrate: 23mL/min Detector: Alltech ELSD

  17. The ELSD 800 does not Respond to Changes in Mobile Phase Composition, Allowing Rapid Separations using Extreme Gradients 1. Aspartame  (0.9mg/mL) 2. Cortisone  (0.3mg/mL) 3. Reserpine  (0.3mg/mL) 4. Dioctyl Phthalate  (0.3mg/mL) 10273 Column: Platinum™ C18, 3µm, 20 x 4.6mm (Part No. 43804) Mobile Phase: A: 0.05% Formic Acid in Water B: Acetonitrile with 0.05% Formic Acid Gradient:(Time,%B),(0,5),(3,90),(7,90),(10,5) Flowrate: 1.0mL/min Detector: Alltech ELSD 800

  18. Near Ambient Temperature Evaporation Maximizes Sensitivity for Semi-Volatiles 1. Glycerol 10268 Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm Mobile Phase: Acetonitrile: 0.04% Ammonium Hydroxide (80:20) Flowrate: 1.0mL/min Detector: Alltech ELSD 800

  19. Mono-, Di-, Tri-, Oligosaccharides 10009 12.  Maltotetraose (DP4) 13.  Maltopentaose (DP5) 14.  Maltohexaose (DP6) 15.  Maltoheptaose (DP7) 16.  Maltooctaose (DP8) 17.  Maltononaose (DP9) 18.  Maltodecaose (DP10) 19.  Maltoundecaose (DP11) 20.  Maltododecaose (DP12) 21.  Maltotridecaose (DP13) 22.  Maltotetradecaose (DP14) 1.  iso-Erythritol 2.  Fructose 3.  Sorbitol 4.  Mannitol 5.  Glucose (DP1) 6.  Inositol 7.  Sucrose 8.  Maltitol 9.  Maltose (DP2) 10.  Raffinose 11.  Maltotriose (DP3) Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: Acetonitrile B: 0.04% Ammonium Hydroxide in Water Gradient:(Time,%B)(0,17),(25,27),(40,45),(60,55) Flowrate: 1.0mL/min Detector: Alltech ELSD

  20. Egg Yolk Phospholipid 10214 1.Phosphatidylethanolamine 2.Phosphatidylcholine Column: Allsphere™ Silica, 3µm, 100 x 4.6mm (Part No. 778382) Mobile Phase: A: Isopropanol:Hexane:Water (58:40:2) B: Isopropanol:Hexane:Water (52:40:8) Gradient:(Time,%B),(0,0),(7,100),(19,100),(20,0) Flowrate: 1.25mL/min Detector: Alltech ELSD

  21. Ginsenosides in Korean Ginseng • 10142 1.  Rh2 2.  Rh1 3.  Rg2 4.  Rg3 5.  Rg1 6.  Rf 7.  Re 8.  Rd 9.  Rc 10.  Rb2 11.  Rb1 Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: Acetonitrile:Water:Isopropyl Alcohol (80:5:15) B: Acetonitrile:Water:Isopropyl Alcohol (80:25:15) Gradient: (Time,%B),(0,25,),(28,85),(35,80),(45,75), (50,90),(51,100),(57,25) Flowrate: 0.8mL/min Detector: Alltech ELSD

  22. 5% Tween 80 • 9017 Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056) Mobile Phase: Water:Methanol (60:40) Flowrate: 1.0mL/min Detector: Alltech ELSD

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