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Explore Mendel's principles of segregation & independent assortment, Morgan's sex-linked inheritance, linked genes, crossing over, and genetic mapping in Drosophila. Decode inheritance patterns and gene sequences on chromosomes.
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Mendel’s “principle of segregation” a.pairs of genes on homologous chromosomes separate during gamete formation (meiosis) and end up in different gametes. b. the fusion of gametes at fertilization pairs genes once again. Ex: The alleles for height in Mendel’s pea plants end up in separate gametes. Tt X Tt could yield four the offspring: TT, Tt, Tt, tt.
Mendel’s “principle of independent assortment” a. each pair of alleles on nonhomologous chromosomes segregates independently of other pairs during gamete formation Ex: When Mendel crossed F1 plants that were heterozygous for round yellow peas, some F2 plants did not resemble the parent plants.
Sex-linked Inheritance w+ = red w = white
Drosophila Testcross Black and Vestigial Wild Type (Gray and Normal) Gray and Normal Black and Vestigial
Evidence for linked genes in Drosophila Non-Parental Phenotype
If the two genes were on different chromosomes, the alleles from the F1 dihybrid would sort into gametes independently, and we would expect to see equal numbers of the four types of offspring. Since most offspring had a parental phenotype it can be concluded that the genes for body color and wing size are located on the same chromosome.
Linked genes tend to be inherited together because they are located near each other on the same chromosome. Recombinants have different phenotypes than the parents.
Morgan, who discovered that genes were located on chromosomes, proposed that some process must occasionally break the physical connection between genes on the same chromosome. This is now called crossing over, which accounts for the recombination of linked genes.
Sturdevant, a student of Morgan’s, devised a way to construct genetic maps, an ordered list of the genetic loci along a particular chromosome. He predicted that the further apart two genes are on a chromosome, the higher the probability that crossing over will occur.
Using recombination frequencies to construct a genetic map a second crossover would cancel out the first and reduce the observed b-vg recomb. frequency 1 map unit is equal to 1% recombination frequency
A genetic map based on recombination frequencies is called a linkage map and is based on the assumption that the probability of a crossover between two genetic loci is based on the distance separating the loci. The distances between genes are expressed as map units, with one map unit equivalent to a 1% recombination frequency.
Partial Genetic Map of a Drosophila Chromosome The numbers represent the distance between that locus and the locus for aristae length.
Investigators performed crosses that indicated bar-eye and garnet-eye alleles are 13 map units apart, scallop-wing and bar-eye alleles are 6 units apart, and garnet-eye and scallop-wing alleles are 7 units apart. What is the order of these alleles on the chromosome?
Given the following recombination frequencies, what is the correct order of the genes on the chromosome? A-B, 8 map units; A-C, 28 map units; A-D, 25 map units; B-C, 20 map units; B-D, 33 map units • A-B-C-D d) B-A-D-C • D-C-A-B e) D-A-B-C • A-D-C-B
The frequency of crossing over between • linked genes A and B is 35 percent; between • B and C, 10 percent; between C and D, 15 • percent; between C and A, 25 percent; • between D and B, 25 percent. The sequence • of the genes on the chromosome is: • ACDB b. ADBC • c. ABDC d. ABCD • e. ADCB
Suppose alleles M and n are linked on one chromosome, and m and N are linked on the homologous chromosome. Individuals homozygous for M and n are mated with individuals homozygous for m and N. Their offspring are crossed with homozygous recessive individuals, and the following results are recorded: Mn/mn 15 mN/mn 13 MN/mn 232 mn/mn 240 How many units apart are these genes on the chromosome?
A wild-type fruit fly (heterozygous for gray body color and normal wings) was mated with a black fly with vestigial wings. The offspring had the following phenotypic distribution: wild type, 778; black-vestigial, 785; black-normal, 158; gray-vestigial, 162. What is the recombination frequency between these genes for body color and wing type?
In another cross, a wild-type fruit fly (heterozygous for gray body color and red eyes) was mated with a black fruit fly with purple eyes. The offspring were as follows: wild-type, 721; black-purple, 751; gray-purple, 49; black-red, 45. What is the recombination frequency between these genes for body color and eye color?
The percentage of crossing-over between four linked genes (K, L,M & N) is as follows: Between M and N: 8 % Between M and L: 19% Between K and N 5 % Between N and L: 11% What is the sequence of genes on the chromosome?
Some Chromosomal Systems of Sex Determination Males have 1 X Sex Chromosome present in ovum Unfertilized males; fertilized females
Although female mammals, including humans, inherit two X chromosomes, one X chromosome in each cell becomes almost completely inactivated during embryonic development. The inactive X in each cell of a female condenses into a compact object called a Barr body, which lies along the inside of the nuclear envelope.
If a female is heterozygous for a sex-linked trait, about half her cells will express one allele, while the others will express the alternate allele.
Calico Cat The white areas are determined by yet another gene.
Klinefelter Syndrome Male with at least 1 extra X chromosome
