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Lab Exercise 14:

Lab Exercise 14:. Biochemical Differentiation of some Medically Important Gram Positive Cocci. Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin).

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Lab Exercise 14:

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  1. Lab Exercise 14: Biochemical Differentiation of some Medically Important Gram Positive Cocci

  2. Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin)

  3. Alpha (α) hemolysis (blood agar around bacterial colonies looks greenish-brown, indicating partial breakdown and consumption of hemoglobin) Mixed culture from a throat swab; note severalβ-hemolytic colonies

  4. Gamma (γ) hemolysis (really means no hemolysis; blood agar around colonies looks normal)

  5. Side-by-side comparisons of alpha (α), beta (β), and gamma (γ) hemolysis

  6. A - Result with no zone of inhibition Bacitracin (Taxo A) sensitivity + results with a zone of inhibition

  7. Optochin (Taxo P) sensitivity - Results with no zone of inhibition + results with a zone of inhibition

  8. P P

  9. Coagulase Test + -

  10. Carbohydrate Fermentation Tests (Glucose and Mannitol) - (same as uninoculated media) + Phenol red indicator turns yellow at acid pH

  11. + - Catalase Test

  12. Staphylococcus epidermidis Enterococcus faecalis

  13. Who am I? M G Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am! Answer: Streptococcus pyogenes

  14. Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am! G M Answer: Micrococcus lutea

  15. Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am! G M A Answer: Enterococcus faecalis

  16. Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am! G M Answer: Staphylococcus aureus

  17. Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am! G M Answer: Staphylococcus epidermidis

  18. G M Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am! Answer: Streptococcus pneumoniae

  19. Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am! G M A Answer: Streptococcus mutans

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