Integrated Fluorescent Probe & Radiofrequency Ablator

1. Integrated Fluorescent Probe & Radiofrequency Ablator Rachel Riti & Alex Walsh Advisors: Bart Masters & Anita Mahadevan-Jansen

2. Background • Determination of ablated tissue area • RFA: Thermocouples • Accuracy • Decreased sensitivity with age http://www.efunda.com/designstandards/sensors/thermocouples/thmcple_theory.cfm?Orderby=Material%20DESC

3. Fluorescence Walsh A, Masters DB, Jansen ED, Welch AJ, Mahadevan-Jansen A, The Effect of Temperature on Fluorescence: An Animal Study, Lasers in Surgery and Medicine. Publishing Pending

4. Objective • Design a probe capable of both fluorescence measurements and RFA • Build the designed probe • Test the probe RITA Medical Systems, “RITA Starburst SDE Electrosurgical Device.” Instructions for Use.

5. Performance Criteria • Maintain RFA efficiency • Determine region of ablation Brainstorming ideas

6. Goals and Contributing Factors • Validate theory • Integrate the fiber optic with adjustable fiber length • Fiber optic length independent of RFA prongs • Limiting Factors: RFA probes, cost of equipment, ability to create viable fibers Masters DB, Walsh A, Welch AJ, Mahadevan-Jansen A, The Effect of Temperature on Fluorescence in Human Tissue (invited paper), In: Optical Interactions with Tissues and Cells XXI. SPIE. Publishing Pending

7. Prototype 1 RFA Probe Fiber optic Laser

8. Validation • RFA functionality not compromised • Fiber functional during RFA • Probe entry not affected • Observable significant difference between ablated and normal tissue • Measurement of ablation area

9. Prototype 2 Fiber enclosed Variable lengths

10. Validation • RFA functionality not compromised • Fiber functional during RFA • Probe entry not affected • Adjustable fiber length during procedure • Fluorescence measurements accurately indicate ablation area

11. Conclusions • Probe 1 • Probe 2