0.1

1. Neighbor Joining Maximum Likelihood BrARF1 BrARF1 99 69 BrARF2-1 BrARF2-1 58 BrARF2-2 BrARF2-2 100 98 BrARF2-3 51 BrARF2-3 Group I Group I BrARF9-1 BrARF9-1 100 100 BrARF9-2 BrARF9-2 99 98 54 BrARF24 BrARF11 69 97 BrARF25 BrARF18-1 95 99 99 BrARF26 BrARF18-2 100 89 98 BrARF24 BrARF27 99 58 BrARF25 BrARF11 99 BrARF26 BrARF18-1 99 100 99 BrARF27 BrARF18-2 BrARF3-1 BrARF3-1 Group II Group II 100 100 BrARF3-2 BrARF3-2 100 97 BrARF4 BrARF4 BrARF5-1 BrARF5-1 51 100 BrARF5-2 BrARF5-3 100 66 65 BrARF5-3 BrARF5-2 54 BrARF7-1 BrARF7-1 99 100 100 Group III BrARF7-2 BrARF7-2 Group III 100 BrARF19-1 99 BrARF19-1 98 99 BrARF19-2 99 BrARF19-2 80 BrARF6 BrARF6 BrARF8-1 BrARF8-1 100 95 91 BrARF8-2 90 BrARF8-2 BrARF10 BrARF10 Group IV 94 BrARF16-1 BrARF16-1 Group IV 100 100 100 BrARF16-2 BrARF16-2 100 BrARF17-1 63 BrARF17-1 100 BrARF17-2 100 BrARF17-2 0.1 0.2 Supplementary Fig. 1. Comparison of the phylogenetic trees of BrARFs. Phylogenetic trees of 31 BrARFs were constructed by the neighbor-joining and maximum likelihood methods with 1,000 bootstrap replicates.

2. Neighbor Joining Maximum Likelihood • AtARF1 AtARF1 100 100 BrARF1 BrARF1 97 • AtARF11 AtARF2 67 44 BrARF11 BrARF2-2 97 • AtARF18 34 BrARF2-1 100 91 66 BrARF18-1 BrARF2-3 94 85 BrARF18-2 AtARF11 100 • AtARF9 BrARF11 99 BrARF9-1 77 AtARF18 100 Group I BrARF9-2 BrARF18-1 74 100 BrARF18-2 • AtARF12 52 44 Group I AtARF9 • AtARF22 100 100 23 BrARF9-1 • AtARF14 33 41 BrARF9-2 • AtARF21 88 55 98 AtARF20 • AtARF15 96 11 AtARF21 93 • AtARF23 53 AtARF15 96 • AtARF20 AtARF22 • AtARF13 89 69 98 AtARF12 BrARF24 58 99 100 AtARF14 BrARF25 86 54 64 AtARF23 99 BrARF26 AtARF13 BrARF27 BrARF24 100 • AtARF2 39 100 BrARF25 100 BrARF2-1 92 BrARF26 BrARF2-2 100 BrARF27 AtARF5 BrARF2-3 AtARF3 99 BrARF5-1 68 Group II 24 100 BrARF3-2 BrARF5-2 24 BrARF3-1 BrARF5-3 63 AtARF4 100 • AtARF7 99 BrARF4 BrARF7-1 100 AtARF5 67 BrARF7-2 97 100 BrARF5-1 33 • AtARF19 100 BrARF5-2 99 50 BrARF19-1 BrARF5-3 BrARF19-2 23 97 AtARF7 50 • AtARF6 Group III 100 BrARF7-1 BrARF6 71 Group III BrARF7-2 99 • AtARF8 AtARF19 100 BrARF8-2 76 BrARF19-1 BrARF8-1 100 100 BrARF19-2 95 • AtARF3 92 AtARF6 Group II 100 BrARF3-1 100 BrARF6 BrARF3-2 82 AtARF8 100 • AtARF4 100 98 BrARF8-2 BrARF4 BrARF8-1 100 • AtARF10 74 AtARF10 52 100 BrARF10 BrARF10 62 100 • AtARF16 Group IV 50 AtARF16 Group IV 99 BrARF16-2 98 BrARF16-2 99 BrARF16-1 BrAFR16-1 100 • AtARF17 100 AtARF17 99 84 BrARF17-1 BrARF17-1 41 BrARF17-2 BrARF17-2 100 0.1 0.1 Supplementary Fig. 2. Comparison of the phylogenetic trees of AtARFs and BrARFs. Phylogenetic trees of 31 BrARFs and 23 AtARFs were constructed by the neighbor-joining and maximum likelihood methods with 1,000 bootstrap replicates.