Work Package 6: Biomolecular motors on nanostructures

1. Objectives Partners PM Midterm-Review Meeting Molecular Machines- Design and Nano-Scale Handling of Biological Antetypes and Artificial Mimics Work Package 6: Biomolecular motors on nanostructures Construct kinesin-motor based biological-inorganic hybrid devices for controlled nano-transport processes. Specific Targeted Research Project (STREP) in FP6-NMP-2002

2. Work Package 6: Biomolecular motors on nanostructures f-actin Myosin Microtubules Kinesin Midterm-Review Meeting The molecular motors How do they work ? Energy conversion (M6-1)Structural changes vs. ATP hydrolysis High resolution techniques How include the motors in nano-devices ? (D6-1)Hybrid organic/inorganic systems Nano-handling Cytoskeleton motors : Kinesin, Myosin...

3. Work Package 6: Biomolecular motors on nanostructures Midterm-Review Meeting Traveling Wave Tracking Detection Objective 60X oil APD

4. Work Package 6: Biomolecular motors on nanostructures Piezo: 10 nanometers steps Single 10 nm step RMS noise ± 2 Å - + Many motors ? Time resolution ~ 2 µs RMS noise ~ 2 Å Drift ~ 1 nm/s - + Single motor ? Midterm-Review Meeting Traveling Wave Tracking

5. Work Package 6: Biomolecular motors on nanostructures T = 300 K kx= 0.3 pN/nm xx = 7.4 pN ms/nm Average speed = 500 nm/s Backward steps ~ 10 % Step time scale < 70 µs Midterm-Review Meeting

6. Work Package 6: Biomolecular motors on nanostructures Midterm-Review Meeting Structures designed with a 3D approach for the TWT setup (collaboration with the Institut Curie) 3D approach Soft Lithography approach In order to observe 3D movements, including rotations, the object of study has to be suspended Glass Some samples

7. Work Package 6: Biomolecular motors on nanostructures Midterm-Review Meeting Nanostructures for kinesin-driven microtubule motility Rectification, confinement and electrical docking Time sequence of microtubule docking

8. Work Package 6: Biomolecular motors on nanostructures Midterm-Review Meeting Rectification of kinesin-driven microtubule motility SEM image of rectifying structure Fluorescence image of microtubule motility in nanostructures Rectifier geometries 92 % efficiency in rectification

9. M6-1 M6-2 and D6-1 Resources Midterm-Review Meeting Work Package 6: Biomolecular motors on nanostructures Van den Heuvel, M. G. L.; Butcher, C. T.; Lemay, S. G.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 235-241. Van den Heuvel, M. G. L.; Butcher, C. T.; Smeets, R. M. M.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 1117-1122. L. Busoni, A Dornier, J-L Viovy, J. Prost, G. Cappello; Journal of Applied Physics 2005, 98 A. Dupont, C. Symonds, L. Busoni, J. Prost, G. Cappello; In preparation (2005) Production Many different nanofabricated structures were made in TU-Delft New flexible optical technique, called Travelling Wave Tracking (ICG-GC). Microtubules injected in the structures (TU-Delft) and the activity has successfully been checked First experiments on single molecular motor Post-Docs: Irene Dujovne, Clémentine Symonds, Lorenzo Busoni Conferences and Meetings New motors: Myosin V and VI (collaboration with J. Spudich, Stanford) Gold nanoprobes (collaboration with R. Levy, University of Liverpool) DNA associated motors additional projects Objectives for the first part of the project have been achieved and we can begin measuring single molecular motors moving on the suspended microtubules. Conclusion Specific Targeted Research Project (STREP) in FP6-NMP-2002