By: Hibah A. W. Abusulaiman

1. Second year Lab Medicine (Females) 1433- 1434 A.H. / 2012- 2013 Microbiology Practical (Course I) • By: Hibah A. W. Abusulaiman

2. Once upon a time… Please don’t sleep

3. Fashionable friends

4. Abrar’s…

5. Sara started the preparation early

6. She got a luxurious manicure and padicure plus…

7. An elegant evening gown

8. Lubna started the preparation 2 days before the wedding

9. She got an elegant evening gown, but…

10. A poor manicure and padicure

11. Both attended the wedding in…

12. After 45 days, Lubna was suffering from…

13. Diagnosed as Hepatitis Patient

14. After discussion with the Doctor, she knew it’s because of the poor hygienic manicure and pedicure

15. Good hygiene Poor hygiene

16. Destruction of Microorganisms

17. So, do you think we are in a war with…?!

18. Simply because they are the agents of… Contamination, infection and decay

19. Early civilization

20. In the 1800s Aseptic techniques invented to prevent contamination of surgical wounds

21. Many organizations classified infection control systems and substances into: • Physical methods. • Chemical methods. Referred broadly as Germicides

22. Germicidal systems fall into three categories: • Decontamination. • Disinfection. • Sterilization.

23. 1. Decontamination: • Reduction of pathogenic microorganisms to be safe to handle without protective attire. • The Lowest level of control. • Includes physical cleaning with soap and detergents and removing of most organic and inorganic materials. • It is the critical step toward disinfection and steilisation.

24. 2. Disinfection: • Disinfectants are liquid chemical agents that kill targeted pathogens but not spores . It can also be solid or gaseous. • It is the next level of control.

25. 3. Sterilization • The complete elimination of viable organisms including spores. • The highest level of control

26. Factors influencing disinfection • Number of microorganisms. • Species and type of microorganism. • Temperature.

28. Physical Antimicrobial Agents • Physical environmental agents can destroy large concentrations of microorganisms in lab cultures or a clinical specimen.

29. Physical Antimicrobial Agents

30. Radiation UV Light To inactivate microorganisms on surfaces

31. Filtration • Filtration is used to remove bacteria, fungi and their endospores from air or solutions.

32. Osmotic Pressure • Extremely hypertonic conditions can cause plasmolysis.

33. Incineration

34. AutoclaveMoist heat- Steam sterilization121OC for 15 min

35. Dry heat160OC for 2 hours OR 170OC for an hour

36. Chemical Antimicrobial Agents • In considering their application to patient care, two general classes: • Those destroying pathogenic microorganisms in the environment (disinfectants) or on skin (antiseptics). • Those administered to patients for treatment of infectious diseases (antimicrobial agents, often called antibiotics).

37. Desinfectants

38. Evaluating a disinfectant • Direct spray method. • Testing the quality of disinfectant from different brands.

39. Testing Disinfectant from Different Brands 0.5 mL 5 mLDettol 5 mL Clorox 5 mL DAC 5 mLGento 5 mL Bacterial Suspension in Nutrient Broth (Control)

40. Testing Disinfectant from Different Brands Water Bath Bacteria in Clorox Bacteria in Dettol Bacteria in DAC Bacteria in Gento Bacterial Suspension in Nutrient Broth (Control)

41. Testing Disinfectant from Different Brands 2 Loopfulls 48 hrs Incubation in the 37O C Water Bath After 5min in Water Bath After 10 min in Water Bath After 20 min in Water Bath Bacterial Suspension in Nutrient Broth (Control)

42. Observe the bacterial growth

43. Objectives • Some infection control terms. • Factors influencing disinfectants. • Physical destruction of microorganisms. • Chemical destruction of microorganisms. • Methods of evaluating disinfectants.

44. Thank you for listeningAny questions?!

45. Thank you for paying attention  Any questions?!