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Quiz #5 (9%) Biol710 11/7/12 name___________. Q1. a) List the three ingredients for a functional plasmid used in cloning: _____ _____ _____ 3%. b) What is the value of a multiple cloning site? ( not needed for plasmid functionality! )
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Quiz #5 (9%) Biol710 11/7/12 name___________ Q1 a) List the three ingredients for a functional plasmid used in cloning: _____ _____ _____ 3% b) What is the value of a multiple cloning site? (not needed for plasmid functionality!) _____________________________________________ 1% c) Draw an expression plasmid (all or none) whereby you can use it for testing enhancers: 2% Q2 You are planning to make a plasmid library to over express unknown proteins using eye cDNA. Your oligo dT primer for reverse transcription will have an EcoR1 site (GAATTC) on it, but now you are deciding between doing a Pac I (TTAATTAA) , a Bgl II (AGATCT) or a Bcl I (TGATCA) digest to open up the coding sequences for fusion into one of the three sets of vectors below. #1 ATGTTAATTAA GAATTC, ATGA TTAATTAA GAATTC, ATGAA TTAATTAA GAATTC #2 ATGAGATCT GAATTC, ATGA AGATCT GAATTC, ATGAA AGATCTGAATTC #3 ATGTGATCA GAATTC, ATGA TGATCA GAATTC, ATGAA TGATCA GAATTC Which set of vectors would you choose? (1%)_____ Give me two reasons why you wouldn’t choose the other two sets. (2%)__________ ___________
