1 / 13

Chymotrypsin

Chymotrypsin. Chymotrypsin is one of the serine proteases. Chymotrypsin is selective for peptide bonds with aromatic or large hydrophobic side chains, such as Tyr, Trp, Phe and Met, which are on the carboxyl side of this bond. It can also catalyze the hydrolysis of easter bond.

kalani
Download Presentation

Chymotrypsin

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chymotrypsin • Chymotrypsin is one of the serine proteases. • Chymotrypsin is selective for peptide bonds with aromatic or large hydrophobic side chains, such as Tyr, Trp, Phe and Met, which are on the carboxyl side of this bond. It can also catalyze the hydrolysis of easter bond. • The main catalytic driving force for Chymotrypsin is the set of three amino acid known as catalytic triad. This catalytic pocket is found in the whole serine protease family.

  2. Properties of an Active Site • A shape that fits a specific substrate or substrates only • Side chains that attract the enzyme particular substrate • Side chains specifically positioned to speed the reaction

  3. The Catalytic Triad

  4. Chymotrypsin Protein Hydrolysis Stage #1

  5. Chymotrypsin Protein Hydrolysis Stage #2

  6. Chymotrypsin Protein Hydrolysis Stage #3

  7. Chymotrypsin Protein Hydrolysis Stage #4

  8. Chymotrypsin Protein Hydrolysis Stage #5

  9. Chymotrypsin Protein Hydrolysis Stage #6

  10. Transition State Stabilization

  11. Chymotrypsin Kinetics The initial "burst" in chymotrypsin-catalysed hydrolysis of the p-nitrophenyl acetate

More Related