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DTG-PCR: A Modified PCR for Solving Traveling Salesman Problem

Explore DTG-PCR, a newly invented tool that modifies the traditional PCR to amplify DNA strands with a lower melting temperature. Discover its operation, effectiveness, and worth through experimental and modeling parts, and consider theoretical aspects. Consider experimental considerations such as different Tms and lengths of DNA strands. Study up-to-date methods like Competitive PCR and Quantitative PCR. Benefit from published studies and computerized simulations. Consider the coalescent approach, Michaelis-Menten equation, and Markov process to enhance your understanding of PCR.

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DTG-PCR: A Modified PCR for Solving Traveling Salesman Problem

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  1. Denaturation temperatuare gradient-polymerase chain reaction: by-product? Ji Youn Lee Cell and microbial engineering laboratory Seoul National University

  2. Let’s talk about DTG-PCR • A newly invented tool by modifying the typical PCR for solving traveling salesman problem • More amplification of DNA strands with lower melting temperature • Is it True? • Does it operate Well? • Is it Worth?

  3. It’s time to study DTG-PCR • Experimental part • Modeling and simulation part • Make use of the published ones : Competitive PCR • Addition of the ssDNA distribution profile with temperature

  4. Let’s think about it!

  5. Experimental considerations • DNA strands with different Tms • Same length, different Tm • or Different length, different Tm • or Different length, same Tm… etc. • Experiments • Mix the strands (two or more) • Perform DTG-PCR in various conditions • Analysis: TGGE, DGGE or graduate PCR

  6. Theoretical considerations • Studies up to date • Competitive PCR • Quantitative PCR • Internal standards and products • Anal Biochem (1993) • Quantitative PCR: Theoretical considerations with practical implications • Replication efficiency E (product and standard)

  7. NAR (1996) • A mathematical model and a computerized simulation of PCR using complex templates • Probability calculation • PNAS (1996) • Efficiency of DNA replication in the PCR • NAR (1997) • A coalescent approach to the PCR • NAR (1998) • Modeling and analysis of competitive RT–PCR

  8. JTB (1997) • Enzymological considerations for a theoretical description of the quantitative competitive PCR • Michaelis-Menten equation (enzyme kinetics) • JTB (1997) • Theoretical description of the PCR • JTB (1999) • Polymerase chain reaction: Markove process approach • Bioinformatics (2001) • Virtual PCR

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