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Fusion format. Ex. BamHI or AttL1. Ex. SalI or AttL2. A. YFG. His. CDS start. CDS stop. Ex. BamHI or AttL1. Ex. SalI or AttL2. B. YFG. Flag. GFP. CDS start. CDS stop. Closed Format. Ex. BamHI or AttL1. Ex. SalI or AttL2. C. YFG. CDS start. CDS stop. Ex. BamHI or AttL1.
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Fusion format Ex. BamHI or AttL1 Ex. SalI or AttL2 A. YFG His CDS start CDS stop Ex. BamHI or AttL1 Ex. SalI or AttL2 B. YFG Flag GFP CDS start CDS stop Closed Format Ex. BamHI or AttL1 Ex. SalI or AttL2 C. YFG CDS start CDS stop Ex. BamHI or AttL1 Ex. SalI or AttL2 D. YFG GFP CDS start CDS stop Ex. BamHI or AttL1 Ex. SalI or AttL2 E. YFG His CDS start CDS stop = start codon = stop codon = relevant CDS sequence