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Recombinant DNA technology

Recombinant DNA technology. DNA isolated from two sources and cut by the same enzyme. Plasmid (vector). Gene of interest. Some uses of genes:. Some uses of proteins:. Recombinant DNA. Gene for pest resistance. Protein for dissolving clots. Gene for toxic-cleanup bacteria.

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Recombinant DNA technology

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  1. Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Plasmid (vector) Gene of interest Some uses of genes: Some uses of proteins: Recombinant DNA Gene for pest resistance Protein for dissolving clots Gene for toxic-cleanup bacteria Protein for “stone-washing” jeans Transgenic organisms Useful products

  2. Recombinant DNA technology Restriction Endonucleases

  3. Recombinant DNA technology First DNA Cloning Experiment Cohen et al., 1973 “Recombinant DNA”

  4. Recombinant DNA technology Cloning Vectors: pBR322 plasmid

  5. Genetically Modified (GM) Foods • In the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified. • ●Corn has been genetically modified to resist insect infestation.

  6. Genetically Modified (GM) Foods Golden Rice

  7. Recombinant DNA technology Palmiteret al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615 “Supermice”

  8. Recombinant DNA technology First Glowing Pet Weeding Out Land Mines

  9. Molecular Techniques •Nucleic Acid Extraction Buffer Components: SDS (sodium dodecyl sulfate) NaCl Sodium citrate EDTA •Polymerase Chain Reaction

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