1 / 33

Development of cDNA Microarrays for Gene Expression Research in Floriculture Crops

Development of cDNA Microarrays for Gene Expression Research in Floriculture Crops. Dave Clark University of Florida Environmental Horticulture Dept. UF Floriculture Biotech Lab (est. 1995). Our main goal: To bridge the gap between basic molecular biology and applied floriculture

katy
Download Presentation

Development of cDNA Microarrays for Gene Expression Research in Floriculture Crops

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Development of cDNA Microarrays for Gene Expression Research in Floriculture Crops Dave Clark University of Florida Environmental Horticulture Dept.

  2. UF Floriculture Biotech Lab(est. 1995) • Our main goal: • To bridge the gap between basic molecular biology and applied floriculture • Florida produces 20% of US floriculture crops

  3. UF Floriculture Biotech Development: • Phase 1 – 1995-2000 (then) • Biotechnology • Phase 2 – 2000-2005 (now) • Functional Genomics • Phase 3 – 2005-2010 (next) • Gene Function

  4. Phase 1 – 1995-2000 • Establish a biotech system • Petunia hybrida • Proof of concept research • Expansion of available technology • Build critical personnel mass • Recruit good people • Make strong collaborations

  5. Phase 1 – 1995-2000 • Transgenic Plants - hormones • Gibberellins • Dwarf plants • Cytokinins • “Stay Green” leaves • Ethylene • Long lasting flowers

  6. Internal Growth Regulators AtGA2ox7 Makes dwarf tobacco Schomburg et al. 2003

  7. Internal Growth Regulators AtGA2ox7 - Makes dwarf petunia

  8. Stay Green Leaves sag-IPT Makes leaves stay green longer in tobacco Gan & Amasino 1995

  9. sag-IPT Makes leaves stay greener longer in petunia Clark et al. in press

  10. sag-IPT – Drought Tolerance

  11. Long Lasting Flowers Etr1-1 ETR1-1 - Makes plants ethylene insensitive

  12. Long Lasting Flowers Control Etr1-1

  13. Problems with 35S::etr1-1 • etr1-1 petunias – commercially limited rooting pathogens – etr1-1 ‘MD’ etr1-1

  14. Conclusions – Phase 1 • We have a good experimental system • We have good people in the lab • We have good collaborators • We needdifferent genes • We needbetter promoters

  15. Phase 2 – 2000-2005 • Functional Genomics • New tools for genetics research • Exploration for new technologies • New and novel genes • Controlling gene expression • Petal specific promoters

  16. Functional Genomics • Tools that help discover the biological function of genes • Uncovers how groups of genes work together in a biological process • Encompasses many traditional biological and genetic approaches

  17. Petunia EST Project (EST = Expressed Sequence Tags) mRNA extraction Tissue collection Bioinformatics and Microarrays library synthesis EST sequencing

  18. Petunia Flower Genes • Petunia gene sequencing: Floral development library 2774 C2H4-treated flower library 3264 Pollinated flower library 1824 Other libraries 1046 • Total genes sequenced 8908

  19. Petunia EST Database

  20. Microarray Construction 4200 unique petunia genes

  21. Microarray analysis – Ethylene Regulated Genes Green A>B Yellow A=B Red A<B Control (A) Ethylene (B) A+B

  22. Ethylene Regulated Genes • We have isolated approximately 50 genes that are regulated by ethylene • Some of these genes are thought to be involved in making floral scent volatiles • Benzoic acid carboxyl methyltransferase

  23. Phase 2 – Still going • We still bridge the gap between basic molecular biology and applied floriculture • We have a petunia EST database • Microarrays are working well • We have many new and novel genes • We have good promoters

  24. Phase 3 – 2005-2010 • Gene Function • We are ahead of schedule: • Experimental system (phase 1) • Adopted by several groups • New genetic tools (phase 2) • Available to floriculture researchers • Understanding gene function

  25. Measuring Floral Scent

  26. Flower Scent Composition - Petunia Benzoic acid carboxyl methyltransferase (BAMT) - makes methyl benzoate in petunia

  27. Stop and Smell the Roses! US consumers bought almost 130 million roses on Valentine’s Day 2003 US consumers spent over $7.5B on cut flowers in 2002

  28. Opportunity Knocks! • Commercial cut rose varieties don’t have much fragrance

  29. Flower Scent Composition - Rose

  30. The Future • We plan to maintain and grow the genetics database • We plan to make transgenic plants expressing new genes • We plan to knock out 1 gene a week – functional analysis • We plan to continue making new collaborations

  31. Who supports us? • Intellectually • Harry Klee – UF • Bill Farmerie – UF • Don McCarty – UF • Michelle Jones – OSU • Jim Giovannoni – USDA/Cornell • EU PetNet Group • Financially • USDA Floriculture Initiative • American Floral Endowment • Fred Gloeckner Foundation • Florida FIRST • The Scotts Company

  32. UF Floriculture Biotechnology2002-2003

  33. Questions?

More Related