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SABLE’S SCOPE

SABLE’S SCOPE. Who We Are. Founded by John R.B. Lighton, Ph.D. 25 years of scientific discovery Tools for understanding – with a respirometry focus Scientists and engineers still active in research Growing at 25% for last 4 years Growing at over 45% in 2011 Fueled by Promethion.

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SABLE’S SCOPE

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  1. SABLE’S SCOPE Who We Are • Founded by John R.B. Lighton, Ph.D. • 25 years of scientific discovery • Tools for understanding – with a respirometry focus • Scientists and engineers still active in research • Growing at 25% for last 4 years • Growing at over 45% in 2011 • Fueled by Promethion

  2. SABLE’S GLOBAL INFLUENCE

  3. Sable’s Promethion Inspiration CUSTOMER REQUESTS: High Throughput User Friendly Scalable No air tight seal Turnkey Solution Good Customer Service Expertise Customizable Analysis Fully Traceable Data No Chemical Desiccants Accurate Fully Integrated Running Wheel Live-in Cages with Bedding

  4. MARKET RESEARCH: Competition The Bad The Good • Small, not live-in cages • No bedding in cages • Very low flow rates (IACUC) • Accuracy concerns • No integrated running wheels • Widely used and cited • Integrated system • High Throughput The Ugly • Black box calculations • No raw data storage or data traceability • Customer service • Difficult to use • Very complex to set up

  5. METABOLIC SCREENING

  6. EXISTING CUSTOMERS

  7. THE CAGE…

  8. FOOD INTAKE Food Intake Monitor • Universal Load Cell • Mouse or Rat hopper Features • 3mg resolution • Crumb tray reduces caching • Real-time measurement Advantages • Increased accuracy, versatility • Fine granularity gives insight into behavioral patterns

  9. FOOD INTAKE Raw Feeding Data • Stores hopper mass vs. time • Recorded to disc at 1Hz One Feeding Episode • Analyze behavior • Determine individual feeding episode intake rates

  10. Summarized Feeding Data FOOD INTAKE • Identify trends and outliers • Can zoom into any event • Automated uptake analysis • Automated meal analysis One Feeding Episode • Analyze behavior • Unprecedented detail available – but only if required

  11. FOOD INTAKE Processed Feeding Data • Outputs cumulative intake vs. time • Shows rate of intake, structure • Can output binned data • Integrates with other cage data • Fully traceable Food Selection • Determine food preference • Control access to either/both foods • Integrates with other cage data

  12. FOOD INTAKE ANALYSIS

  13. FOOD ACCESS CONTROL Access Control Module • Access Control Door • Connects to mass sensor Features • Computer controlled access to food • Available for Mouse or Rat food hoppers • Intelligent Obstruction Detection Advantages • Reduced technician interaction • Customizable assays (paired/yoked)

  14. FOOD ACCESS CONTROL User Friendly Setup • Select from 8 preset assays • Customize time and duration • Individual cage setup Select Access Control Type • Add to your custom assay • Unlimited number of variations

  15. WATER INTAKE Water Intake Monitor • Universal Load Cell • Mouse or Rat dispenser models Features • 3 µL resolution • Real-time measurement • Stainless steel case (not shown) reduces animal interaction • Custom sipper tube reduces leakage Advantages • Increased accuracy • Increased granularity gives insight into behavioral patterns • Use existing sterilizing systems

  16. WATER INTAKE Raw Drinking Data • Stores bottle mass vs. time • Recorded to disc at 1Hz Processed Drinking Data • Analyze behavior • Mathematical drift elimination • Determine individual episode intake rates • EthoScan Compatible

  17. BODY MASS Body Mass Monitor • Universal load cell • Mouse or Rat models available Features • 3mg resolution • Real-time measurement Advantages • Highly accurate • Provides in-cage enrichment • Reduced technician interaction

  18. BODY MASS Raw Body Mass Data • Stores bracket mass vs. time • Recorded to disc at 1Hz • Is used about every 15 minutes Zoomed In • Track body mass over time

  19. BODY MASS Processed Body Mass Data • Stores most recent body mass for each timestamp • Synchronized with other data • Diurnal cycle clearly visible

  20. VOLUNTARY EXERCISE Wheel-M or Wheel-R • Mouse or Rat Wheel • Magnetic Reed Switch • Optional: Wheel Stop Features • Real-time measurement • Stainless steel construction • Entirely in cage Advantages • Increased granularity gives insight into behavioral patterns • Correlates with metabolic data

  21. VOLUNTARY EXERCISE Raw Running Wheel Data • Stores RPS vs. time • Recorded to disc at 1Hz Processed Running Wheel Data • Shows cumulative distance run • Can be binned and synchronized with metabolic data

  22. TOTAL ACTIVITY BXYZ-M or BXYZ-R • XY and Z IR arrays • Mounting bracket Features • 0.25cm calculated centroid • Real-time measurement • Intelligent obstruction detection • Rearing captured with Z axis Advantages • Highly accurate • Not affected by static obstructions • Correlated with metabolic data

  23. TOTAL ACTIVITY Activity Analysis • Total Activity • Total Distance Traveled • Rearing • Activity associated with in-cage devices • In cage position vs. time • Customizable assays • Position histograms Activity Comparison • WT vs. ob/ob • Interaction with FH-MB • Time spent time spent near food hopper

  24. CALORIMETRY Flow Generators & Gas Analyzers • Automated calibration • Sequential or continuous monitoring • Can group for 4, 8,16, 24, 32 etc. cages • Expandable and modular • Water vapor dilution correction • Integrated O2, CO2, WVP analyzers • Pull-mode (negative pressure)

  25. DATA ANALYSIS Data Synchronization • Overlay metabolic data with other continuously monitored parameters Further Analysis • All data are perfectly synchronized and can be exported to other programs if required (open formats)

  26. DATA ANALYSIS Further Data Synchronization • Body mass and food intake • Body mass and water intake • Body mass, food intake, water intake, and wheel revolutions Advantages • Synchronized data yields traceability • Real time measurement improves visibility • Both enable behavioral monitoring

  27. DATA ANALYSIS Multiplexed vs. Continuous • Continuous measurements give unprecedented detail • Multiplexed measurements are adequate for many research topics • You have the choice

  28. TOTAL ACTIVITY Activity Analysis (II) • Activity within user-defined areas of any shape • Regions of interest interactively defined on customizable background • Correlate with other data streams as needed

  29. TOTAL ACTIVITY Activity Analysis (III)

  30. TOTAL ACTIVITY Activity Analysis (IV)

  31. DATA ANALYSIS

  32. DATA ANALYSIS

  33. DATA ANALYSIS

  34. Multiplexing errors- more information

  35. Error from multiplexing

  36. Over and underestimates

  37. DATA ANALYSIS Raw Data Synchronization - Zoomed • Body mass (blk), food intake (red), wheel (yel), and water intake (blu)

  38. ETHOSCAN Behavioral Monitoring • All cage devices continuously monitored (“focal animal”) • Interactions sequentially processed in data analysis • Output is timestamp, position, duration, amount and activity

  39. ETHOSCAN Behavioral Transition Matrix • Statistical analysis of interaction with in-cage devices • Shows probability of behaviors in the top row happening immediately after the behavior in the leftmost column

  40. ETHOSCAN Time Budgets • Shows percentage of time spent exhibiting a specific behavior • Customizable to your cage setup and behaviors you wish to monitor Ambulatory Budgets • Two wild type mice –with and without access to a wheel • Note the significant difference in distance travelled

  41. ROOM CALORIMETRY

  42. ROOM CALORIMETRY • Time-consuming to set up and test • Just one span per run… But analyzers drift • Propane burn validations may require back-calculation of span gases (black box!) • Cannot adequately account for water vapor dilution, especially at low WVP • System integration difficult • Many individual instruments = many failure points and many companies to deal with • Little or no expandability or modularity

  43. ROOM CALORIMETRY

  44. ROOM CALORIMETRY • Raw data not preserved, so - Not auditable • Comparisons between systems problematic • Little emphasis on traceability (pre-Jon Moon) • But – traceability alone will not help • Also need transparency! • Custom “black box” software is proprietary • Software is a priori optimized for distinguishing between two activities

  45. ROOM CALORIMETRY • Highly stable O2, CO2 analyzers required • Low noise (for response correction) • WV dilution is a problem… • Chemicals are impractical… • Typically, chilled condensers are used • But these only “dry” the air to ~650 Pa WVP • At dewpoints < 1 C, they have no effect at all • Plus, FiCO2 changes…

  46. ROOM CALORIMETRY FiCO2 changes…

  47. ROOM CALORIMETRY + • Minimize O2 drift (SSI O2 analyzers) • Then, eliminate O2drift by repeated, interleaved FiO2 measurement (PP) • For CO2, use interleaved SSI analyzers (PP) • For eliminating WV dilution, don’t scrub/condense – calculate from WVP, BP! (PP) • Record all raw data in complete detail

  48. ROOM CALORIMETRY + • Use computational processing to reassemble the dry O2 and CO2 data from the interleaved O2, CO2, WVP data, plus BP (PP) • Apply rigorously neutral smoothing and response correction to data • All using a traceable, auditable, shareable script • Because raw data are stored, any kind of response correction you wish can be applied!

  49. PROMETHION

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