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SMTP Lab Safety Briefing (SMTP and Cat 1 Students). General. Students can only work in the lab in the presence of a teacher or lab staff. Booking of equipment and requests for materials must be made to at least 3 days prior to experiments, with mentor kept informed.
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General • Students can only work in the lab in the presence of a teacher or lab staff. • Booking of equipment and requests for materials must be made to at least 3 days prior to experiments, with mentor kept informed. Mdm Lim: Bio lab (limcf@hc.edu.sg) Mdm Xia: Chem lab (xiaying@hc.edu.sg) Mdm Chua: Physics/Photonics • SRC Tel. no.: 64680379
General rules • Please keep lab tidy at all times. • Spills must be cleaned immediately to avoid contamination. • Gloves must be worn when handling hazardous materials. • Chipped or broken glassware should never be used. Please give these to lab staff for disposal.
General rules • Centrifuges must be balanced before operation.
Scientific Review Committee (SRC) Approval • Apply for approval (through your mentor) from the Scientific Review Committee if your project involves any of these: • Potentially hazardous biologial agents (e.g. bacteria) • Vertebrates
Institutional Review Board (IRB) approval • Institutional Review Board (IRB) approval required for studies involving • Human Subjects • An IRB must include • A science educator • A school administrator (Principal or VP) • A medical doctor, physician’s assistant, registered nurse, psychiatrist, psychologist, licensed social worker or licensed clinical professional counsellor
SRC Approval • Approval from must be submitted to Mrs Goh Cheng Wai before the commencement of your projects ( see Mrs Goh’s EMB msg) • SMP groups will submit forms to Mr Chia KP
Research Projects • Students working in Research labs must wear a lab coat. These should be folded and placed on designated shelves when not in use (ref. Lab staff). • In bio labs, laminar hoods --- perspex shield must be in place if UV light is turned on.
Microbiology Projects • All microorganisms must be treated as potential human pathogens. • Only Biosafety Level 1 organisms can be used. • Microorganisms should not be isolated from potentially hazardous sources e.g.s polluted water, human mucus, pus and faeces • All microbial cultures and culture media must be labelled: date, name of student & teacher.
Microbiology Projects • BSL1: low risk to individual and the community • BSL2: moderate risk to individual but low risk to the community. Can cause diseases in human but unlikely to be serious hazards to lab users under normal circumstances. • BSL3: high risk to individual but low risk to community. Usually cause diseases in human, treatable with anti-microbial agents, not contagious. • BSL4: high risk to individual and community. Usually cause serious diseases in human, untreatable and contagious.
Prohibited work Studies involving: • Fresh tissue, blood or body fluids from humans or vertebrates. • Human or animal blood or blood products. • Human or vertebrate/mammalian cell tissue cultures
Approved work Studies involving: • Plant tissue • Cheek cells (but not cultures) • Meat or meat by-products obtained from food stores, restaurants or packing houses. • Hair • Sterilised teeth
Sharps bins Disposal of sharps • Sharps --- needles, scalpel blades, micropipette tips, glass slides, cover slips
Decontamination • Autoclave or bleach all cultured organisms esp. microbes before disposal • Disinfect work surfaces if microbes were used --- swab with 70% alcohol
Reminder • Young Explorers’ Fund (YEF) • Capped at $2000 a group • Can apply any time – submit forms to mentors • Forms on SMTP webpage
Risk Assessment SMTP Research Session
Risk assessment • Risk assessment is needed for all Cat 1 projects • To be completed by students, reviewed and approved by mentor. • Before experiments begin, risk assessment to be submitted to SRC - Mdm Lim (for bio projects) - Mdm Xia (for chem projects) - Mdm Chua (for phy projects) • SMTP students will complete risk assessment together with proposals. Mentor to submit to above personnel after he/she approves.
Risk Assessment Documents • SRC Equipment Risk Assessment • Risk assessment form Both forms will be available from SMTP wiki.
How to complete risk assessment? • For risk assessment of equipment, refer to resources\SMTP wiki • For chemicals, search for MSDS online, look for info on possible hazards and measures to reduce the risks. • For micro-organisms, check out on - biosafety level of bacteria - possible infection - handling precautions
Useful sites for biology projects • http://academic.marion.ohio-state.edu/sciencefair/BSL.htm • http://www.sciencebuddies.org/science-fair-projects/project_ideas/Micro_Safety.shtml • http://www.sciencebuddies.org/science-fair-projects/project_src_biological_agents.shtml • http://www.sciencebuddies.org/science-fair-projects/project_src_vertebrate_animals.shtml