E N D
Synthetic Oligo X, Y = Any nucleotide NdeI N term 5’XXXX ATGAAGAGTACATTTTCAGTCATCTACTACCTCAAGCGTCAGGTAGTGAAAAAGGACGGGACAGTTCCCGTCATGGGACGCATCACGGTGGACGGCAGCCAGACGCAGTTCAGCTGCAAACTGACTGTCGACCCGAAGCTGTGGGACACCAAAGGGGGACGTGTCACGGGCAGAAGCACGGCGGCACTTGAAACGAATCGTATGCTTGACAAGATGCGGGTGCGCATCAACAAGCACTATCAGGAAATCATGGAGCGTGACAACTTCGTCACGGCGGAGAAGGTGAAGAACGCCTTTCTCGGATTGGAACACCGCTACCACACGCTGATGCAGGTGTTCCGCCGGCACAACGAGGACTATGAGAAACAGGTGGAGGCTGGCATGAAAGCCAAAGGCACGCTGTTGAAGTACCGCACCGTTTACAAGCACCTGCAAGAGTTCCTCACCATCCGCTACCATGTGAAGGACATCGCCCTGAAAGAGCTTACCCCGGCTTTCATCTCCGACTTCGAGATGTTCCTGCGCACGGACAAGCACTGCTGCACCAATACCGTGTGGCTGTACGTCTGCCCGCTACGGACGATGGTGTTCATCGCCATCAACAACGAGTGGCTGACACGCGACCCGTTCCGCGAGTATGAAATCAAAAAGGAAGAAACGACACGCAGTTTCCTTACCAAAGAGGAAATACGCCTGTTGATGGAAGGCAAACTGAAAAACGCCAAACAGGAACTGTACCGCGACCTCTACCTGTTCTGCGCCTTCACCGGATTGTCATTCTCGGATATGCGCAACCTCACGGACGAGAATATCCGTACCTACTTCGACGAACACGAGTGGATAAACATCAAGAAAACGGGTGTGGTGTCCAATATCCGCCTGCTTGACATCGCCAACCGCATAATCGGCAAATACCGGGGACTGTGCGGGGACGGCAGGATATTCCCCGTCCCGCATTATAACACGTGCCTTGCAGGTATCCGTGCCGTCGCCAAGCGTTGCGGCATCACCAAGCATATCACGTGGCATCAAAGCCGCCACACGGCAGCCACGACAGTTTTCCTTTCCAACGGCGTACCCATCGAAACGGTCAGCTCCATGCTCGGACACAAGAGCATAAAGACGACGCAGATTTACGCGAAGATAACCAAAGAAAAGCTCAATCAGGACATGGAGAACCTTGCGGCAAGGTTGAACGGCATCGAGGAATTTGCAGGTTGTACCATCTAA Stop 3’ BamHI C term CCTAGGTTTT