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BIPN 148 Lecture 15

BIPN 148 Lecture 15. Calcium regulation of gene expression. PO 4. PO 4. The CREB/CRE Gene Regulation System. acetylation. pKAF. Histone. CREB Binding Protein (CBP). RNA Helicase A. ser 133. ser 133. RNA Polymerase II. Basal Transcription Factor IID (TFIID) Complex. CREB. CREB.

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BIPN 148 Lecture 15

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  1. BIPN 148 Lecture 15

  2. Calcium regulation of gene expression

  3. PO4 PO4 The CREB/CRE Gene Regulation System acetylation pKAF Histone CREB Binding Protein (CBP) RNA Helicase A ser 133 ser 133 RNA Polymerase II Basal Transcription Factor IID (TFIID) Complex CREB CREB Target Gene cAMP Response Element 5’-TGACGTCA-3’ TATA Sequence

  4. CREB phosphorylation in fear conditioning

  5. CRE-mediated gene expression in fear conditioning

  6. CREB and taste aversion

  7. CREB and Fear Conditioning

  8. Transcriptional targets of CREB CaMKIV + ERK RSK2 Elk-1 CBP/CREB zif268 CRE SRE C/EBP krox20 Arc AMPAR HOMER BDNF NT-3

  9. Synaptic Tagging and LTP New gene products or proteins New gene products or proteins Synaptic tag NMDAR Signal to nucleus Synaptic Potentiation Locally generated tag captures new gene product

  10. THE CASE FOR GENE EXPRESSION IN L-LTP EXPERIMENT TYPE and FINDING Block Block of L-LTP with protein/RNA synthesis inhibitors  Block of L-LTP with RNA synthesis inhibitors Loss of L-LTP with CREB KOs Block of L-LTP with Arc antisense Loss of L-LTP with CaMKIV KO  Loss of L-LTP with zif268 KO

  11. THE CASE FOR GENE EXPRESSION IN L-LTP EXPERIMENT TYPE and FINDING Measure Increased zif268/krox24 mRNA  Increased krox20  Increased expression of fos, jun IEG mRNAs Increased CREB phosphorylation Increased CRE read-out Increased elk-1 phosphorylation  Increased Arc/Arg3.1 mRNA expression Increased AMPA receptor protein  Increased BDNF message 

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