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Chapter 17. Viruses & prokaryotes. 17-1 Viruses. What is a virus? How do viral life cycles differ? What is the relationship between viruses and their hosts?. What is a virus?. A virus is a noncellular particle made up of genetic material and protein that can invade living cells
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Chapter 17 Viruses & prokaryotes
17-1 Viruses • What is a virus? • How do viral life cycles differ? • What is the relationship between viruses and their hosts?
What is a virus? • A virus is a noncellular particle made up of genetic material and protein that can invade living cells • First discovered in 1935 by an American scientist named Wendell Stanley • It was called the tobacco mosaic virus or TMV
TMV on tobacco plant http://healthyhomegardening.com/Disease.php?pid=15
http://concerncrisis.blogspot.com/2008/03/tobacco-mosaic-virus-tmv.htmlhttp://concerncrisis.blogspot.com/2008/03/tobacco-mosaic-virus-tmv.html
Structure of a virus • Composed of a core of DNA or RNA surrounded by a protein coat called a capsid • Capsid protects the genetic material • The core contains several genes to several hundred genes
More complex structures are in viruses called bacteriophages – viruses that invade bacteria • Has a head region (capsid), a nucleic acid core, and a tail • Some have tail fibers that allow them to attach to the bacteria
Viruses are rod-shaped, tadpole-shaped, helical and cubelike shaped • Vary in size from 20 to 400 nanometers • A nanometer is one billionth of a meter
http://learnsomescience.com/microbiology/viruses-viroids-and-prions/http://learnsomescience.com/microbiology/viruses-viroids-and-prions/
Specificity of a virus • Specific viruses infect specific organisms • Plant virus cannot infect an animal • Only mammal viruses that do not infect other animals and vise versa • Viruses for every type of organism
http://www.wellesley.edu/Chemistry/Chem101/dna-viruses/viruses.htmlhttp://www.wellesley.edu/Chemistry/Chem101/dna-viruses/viruses.html
Life cycle of a Lytic Virus • In order to reproduce, viruses must invade, or infect, a living host cell • They also invade in different ways… • One way is done by lytic viruses where when they invade the cell bursts or lyses
Life cycle of a lytic virus http://dvm5.blogspot.com/2010/11/classification-of-viruses.html
Infection • A virus is activated by contact with the right host cell (chance) • It then injects its DNA into the cell
Life cycle of a lytic virus http://dvm5.blogspot.com/2010/11/classification-of-viruses.html
Growth • The RNA polymerase of the host cell creates messenger RNA of the virus DNA • This mRNA then takes over the host cell • Some proteins turn off the creation of molecules for the cell
Life cycle of a lytic virus http://dvm5.blogspot.com/2010/11/classification-of-viruses.html
Replication • It then uses the host cell to make thousands of copies of its own protein coat and DNA • The host cell is then filled with viral DNA molecules • These three steps can happen in 25 minutes
Life cycle of a lytic virus http://dvm5.blogspot.com/2010/11/classification-of-viruses.html
The infected cell then lyses (bursts) and releases hundreds of virus particles • These particles than infect other cells • The host cell is lysed and destroyed so this process is called lytic infection
Lysogenic Infection • Lysogenic infection-the virus does not reproduce and lyse its host cell • The DNA of the virus enters the cell and is inserted into the DNA of the host cell • The viral DNA is then known as a prophage
Prophage activity • Blocks entry of other viruses and may even add useful DNA to the host cell’s DNA • Eventually it will remove itself from the DNA and create new virus particles
Retroviruses • Retroviruses contain RNA as their genetic information • When they enter the cell they produce a DNA copy • This then enters into the host cells DNA
http://ucdbiotech.wordpress.com/2009/10/13/be-on-the-alert-the-first-ever-gammaretrovirus-capable-of-infecting-human-hosts-has-been-identified/http://ucdbiotech.wordpress.com/2009/10/13/be-on-the-alert-the-first-ever-gammaretrovirus-capable-of-infecting-human-hosts-has-been-identified/
Viruses and Living Cells • Viruses must infect living cells to carry out their functions • Viruses are parasites-an organism that depends entirely upon another living organism for its existence in such a way that it harms that organism
http://westernrifleshooters.blogspot.com/2011/02/interview-with-stanley-kurtz.htmlhttp://westernrifleshooters.blogspot.com/2011/02/interview-with-stanley-kurtz.html
Are viruses alive? • Viruses are not made of cells • They can grow, reproduce, regulate gene expression, an evolve • It is up for debate
17-2 Prokaryotic Cells Bacteria Intro video http://hyperphysics.phy-astr.gsu.edu/hbase/biology/imgbio/cellprokaryote6.gif
Prokaryotes • Cells that do not have a nucleus http://en.wikipedia.org/wiki/Prokaryote
Eubacteria http://calexa.weebly.com/eubacteria.html
Cyanobacteria http://user.uni-frankfurt.de/~schauder/cyanos/cyanos.html
Archaeabacteria • Methanogens – archaeabacteria that produces methane gas http://en.wikipedia.org/wiki/Archaea
Prochlorobacteria http://jk169.k12.sd.us/proteobacteria.htm
Bacteria (E. Coli) • One-celled prokaryotes http://en.wikipedia.org/wiki/Bacteria
They do not contain the complex range of membrane enclosed organelles that are found in most eukaryotic cells http://www.weblife.org/humanure/images/fig3-1.jpg
Eubacteria Structure • generally surrounded by a cell wall made of carbohydrates • there is a cell membrane which surrounds the cytoplasm • long whip like flagella protrude from the membrane through the cell wall
Eubacteria http://upload.wikimedia.org/wikipedia/commons/thumb/5/5a/Average_prokaryote_cell-_en.svg/494px-Average_prokaryote_cell-_en.svg.png
They use photosynthesis to get energy http://www.biologie.uni-hamburg.de/b-online/library/webb/BOT311/Cyanobacteria/CyanoHyellaStella300Crop.jpg
Fresh and saltwater, land, hot water, arctic, grow on snow http://lyxia.us/wp-content/uploads/2012/08/cyanobacteria-1.jpg
Methanogens are archaebactria that produce methane gas http://www.daviddarling.info/images/methanogens_large.jpg
prochlorobacteriamore related to chloroplasts http://jk169.k12.sd.us/images/Escherichia_coli1355024.300a[1].jpg
Bacteria Identification • cell shape • Cell Wall • Bacterial Movement • How the obtain energy
Bacterial shape https://benchprep.com/blog/ap-biology-diversity-of-organisms-kingdoms-part-i/
Gram Staining • There are two types of dye, The bacterial cells with only one thick layer of carbohydrate and protein molecules outside the cell membrane took up the crystal violet. The bacterial cells that have lipid and carbohydrate molecules appear red under the microscope and are gram negative
Gram Staining http://en.wikipedia.org/wiki/Gram_staining