Figure S2. Expression patterns of OsAHP1 and OsAHP2 .

1. Figure S1. The coding sequence alignment among OsAHPs and OsPHPs, and the relative expression of OsAHPs and OsPHPs in OsAHPs-RNAi and wild-type rice seedlings. (A) Schematic representation of the OsAHP-RNAi construct. The numbers indicate the nucleotide positions in the OsAHP2 coding sequence. Pubi, maize Ubiquitin promoter. (B)The coding sequences of the genes were aligned; underlining indicates the RNAi fragment used to knock down OsAHP1-2. (C) Measurement of the mRNA levels of two OsAHPs and three OsPHPs in shoots and roots by qRT-PCR.

2. Figure S2. Expression patterns of OsAHP1 and OsAHP2. (A) Real-time PCR analysis of OsAHP1 and OsAHP2 expression in different tissues. (B-H) GUS staining assay of ProOsAHP2::GUS transgenic rice plants. (B) Five-day-old seedlings. (C) Five-day-old seminal roots. (D) Eight-day-old seminal roots and lateral roots. (E) Lamina joint. (F) Mature leaf. (G) Basal node. (H) Spikelet.

3. Figure S3. OsAHP2 overexpression partially rescued the Arabidopsis ahp1,2,3,4,5mutant phenotype. (A) Nine-day-old seedlings of wild-type, the ahp1,2,3,4,5 quintuple mutant, and ahp1,2,3,4,5 mutant expressing the OsAHP2-GFP fusion protein grown under long-day conditions. (B) Confocal image of the OsAHP2-GFP fusion protein in root cells of the transgenic plant shown in (A). (C) Western blot analysis of OsAHP2-GFP expression in two lines of seedlings shown in (A) using anti-GFP antibodies. Rubisco (RBC) was used as a loading reference.

4. Supplemental Table S1. Primers used for gene cloning.

5. Supplemental Table S2. Primers used for qRT-CR analysis.

6. Coin Figure: Thirty-day-old wild-type (WT) and OsAHPs-RNAi seedlings grown on 250 mM mannitol-containing mediums