Advancements in Molecular Diagnostics and Mitochondrial Research Laboratory

1. 2011년 연말 정리 화순전남대학교병원 미토콘드리아 연구실 (Molecular Diagnostics and       Mitochondrial Research Laboratory) 홈페이지주소- http://www.mtdna.or.kr 2011. 12. 23 (금)

2. Molecular Diagnostics and Mitochondrial research laboratory Treatment Outcome Hematopoietic Stem cells Hematopoietic Ds MRD determination Biomarkers Chronic Degenerative Diseases Potential new therapeutics New molecular Pathophysiology Cancer/Aging Diagnosis

3. 정리 내용 • 연구원 동정 • 학술대회 참가 및 초록발표 • Accepted manuscript • 준비중인 SCI논문 • 연구과제 수행

4. 2011년 MTDNA연구팀실험실 동정 • 2011.05.27-28 대한혈액학회 우수연제상 수상(서울 그랜드힐튼호텔) • 2011.06.03 2011소아암환경보건센터 세미나 개최 (경희대 의대 김원교수님) • 2011.06.11-12 화순전남대병원 소아암환경보건센터 심포지움 (변산대명리조트) • 2011.06.20 김민영 연구원 한국병원 입사 • 2011.06.22 융복합기술기반 신속통합진단 시스템 심포지엄 개최 (화순전대병원) • 2011.07.21 미토콘드리아 실험실 2011년 2분기 모임 (해남 세브란스의원 방문) • 2011.7.26-7.29 김혜란박사, 김환영연구원 BD FACSAria II training course 참석 (BD Singapore office) • 2011.09.03 미토콘드리아 실험실 담양금성산성산행 • 2011.9.30 라만레이저 응용 의료진단기술 심포지움(전남대학교 용봉캠퍼스공대) • 2011.10.01 원스테파니 실험실 연구원 시작 • 2011.10. 22 미토콘드리아 실험실 전북 완주 대둔산 산행 • 2011.11.13 미토콘드리아 실험실 장흥 천관산 산행 • 2011.11.23 화순전남대병원 소아암 환경보건센터 심포지움 (화순전남대학교 병원) • 2011.11. 10 고정윤 학생 대학원 석사 합격 • 2011.12.02 IT융복합연구회 송년 심포지엄 (전남대학교 용봉캠퍼스공대) • 2011.12.10-13 신명근교수님, 김혜란박사 미국혈액학회 참석(San Diego, CA)

5. 2011 학술대회 참가 및 초록제출 1) Korea-Germany International Joint Symposium- 2011.03.04 화순전남대 병원 대강당 ‘Molecular diagnostics and mitochondrial research in cancer and chronic inflammatory diseases’ 2) 미토콘드리아허브제어연구센터 초청강의 - 2011.03.24 부산 동아의대 ‘Translational Mitochondrial Research in Hematopoietic and Chronic Inflammatory Diseases’ 3) 2011 IGCC, 서울 코엑스 (2011. 4.20-23) - Mitochondrial genome instability in barrett's esophagus associated with increased production of reactive oxygen species 4) 2011 ISLH, New Orleans, Louisiana (2011.5.6-8) - Mitochondrial DNA copy number and hnRNP A2/B1 protein are biomarkers for benzene itself-related toxicity and hematotoxicity - Metformin selectively kills the AML stem cells through increased phosphorylation of AMP-activated protein kinase - The spectrum of mitochondrial genome instability in acute myeloid leukemia and leukemia stem cells

6. 5) The 52nd Spring Meeting of the Korean Society of Hematology– 서울 그랜드힐튼호텔(2011.5.27-28) - Mitochondrial DNA Minisatellites Markers in Determination of Chimerism after Allogeneic SCT - Mitochondrial Genome Instability is Frequently Occurred in Acute Myeloid Leukemia Stem Cells (우수 연제상 수상) - Serum Fibrin Degradation Product is a Useful Biomarker for Diagnosis and Disease Monitoring in Multiple Myeloma - Dose Serum Free Light Chain Ratio Predict Prognosis for Newly Diagnosed Multiple Myeloma? - Increased Mitochondrial Mass and Mitochondrial DNA Copies are Biomarkers for Benzopyrene Exposure in Blood Cells and Hematopoietic Tissues - Effects of Storage Temperature, Type of Anticoagulant and Cryopreservation on Human Peripheral Blood Mononuclear Cells for the Assessment of NK Cell Cytotoxicity

7. 6) 2011 Siemens Customer Seminar – 2011.06.15 광주 라마다 프라자 호텔 ‘Update Reciew, Clinicopathological Utility and Experience of Serum Her-2/neu Measurement’ 7) 융복합기술기반 신속통합진단 시스템 심포지엄 – 2011.06.22 화순전남대병원 대강당 ‘융복합 관점에서 신속진단검사 시스템 개발 필요성’ 8) Abbott Scientific Symposium- 2011.07.12 광주 라마다 프라자 호텔 ‘ProGRP is sensitive and specific tumor markers for small cell lung cancer’ 9) 유전 및 분자진단연구회 2011년 학술대회 - 2011.07.15 ‘Distinctive hematological abnormalities in East Asian neonates and children with down syndrome’ 포스터발표 10) 대한진단검사의학회 제 52차 학술대회– 무주 덕유산리조트(2011.10.06-07) - Cancer Stem Cell in Hematological Malignansies - Comparison of Clinical and Laboratory Standards Institute(CLSI) and European Committee on Antimicrobial Susceptibility Testing(EUCAST) Broth Microdilution Methods for Testing Fluconazole Susceptibility of candida Bloodstream Isolates by Use of Epidemiological Cutoff Values - Mitochondrial DNA Minisatellite Markers have Enough Power for Discriminating Chimerisms after Allogeneic Stem cell Transplantation - Remarkble Difference of Serum CK-MB value According to methods between Immunoinhibition and Immunoassay

8. - Dysfunction of Natural Killer T cells in Patients with Active Mycobacterium tuberculosis Infection - Interleukin-21 Enhances Cytotoxicity of ex vivo Expanded NK cells against Breast Cancer cells - Cytogenetic Profiling of Acute Leukemia in Korean patients and Implication for the Development of a New Molecular detection system - Constitutional t(7;14)(p15';q11) in a young Woman Manifesting only Myelodysplastic syndrome - Serum free light chain and its ratio are useful Serum Biomarkers for Predicting Prognosis and Assessment of risk Stratification in Multiple Myeloma - Microbiological Characteristics of Clinical isolates of Candida auris - Constitutional del(9)(p13) is Associated with Mediastinal large B-cell Lymphoma without the consensus Phenotype of Deletion 9p syndrome - High-Frequency Minisatellite Instability in the Mitochondrial Genome in Primary Colorectal Cancer Tissue is Associated with Clinocopathological Parameters - Effects of Storage Temperature, Type of Anticoagulant, and cryopreservation on Human Peripheral Blood Samples for the Assessment of Natural Killer Cytotoxicity

9. 11) 2011 라만레이저 응용 의료진단기술 심포지움 – 2011.9.30 전남대학교 용봉캠퍼스 공대 ‘융복합 신속진단 연구주제 발굴 사례’ 12) 제61회 대한해부학회 - 2011.10.20 부안 대명리조트 ‘Serum complement factor H and apolipoprotein H - novel molecular indicators of remission status in patients with acute myeloid leukemia ’ 13) 4th Annual Asia Pacific&Japan Scientific Symposium-2011.10.27-28 태국 방콕 ‘Plasma ProGRP concentration is Sensitive and Specific for Discriminating Small Cell Lung Cancer’ 14) 2011 Hwasun optical imaging workshop & symposium - 2011.11.02 ‘Kick-off Symposium of Bacteriotherapy Consortium’ 15) 2011년 소아암 환경보건센터 심포지움 – 2011.11.23 화순전남대학교 병원 ‘Mitochondrial biomarkers for benzene and benzopyrene exposure’ 16) 2011 국제노인과학 연합학술대회 – 2011.11.25 서울시립대학교 ‘Aging of hematopoietic stem cells and implications in hematological disorders’   17) 금강포럼- 2011.12.03 서울아산병원 ‘Engraft Chimerism and MRD Detection’ 18) 2011 ASH Annual Meeting– 2011.12.10-13 San Diego, CA - Mitochondrial DNA Minisatellites Showed Higher Informativeness and Sensitivity Than the Nuclear DNA Markers for the Quantitative Determination of Chimerism After Allogeneic Stem Cell Transplantation - Biomarker for benzopyrene exposure is mitochondrial mass and mitochondrial DNA copy in blood cells and hematopoietic tissues - Distinctive spectra of cytogenetic alterations in over five hundred East Asian patients with acute leukemia

10. 2011 ACCEPTED MANUSCRIPT • 주저자 및 교신저자 • 1) Plasma proGRP concentration is sensitive and specific for discriminating small cell lung cancer from nonmalignant conditions or non-small cell lung cancer. (주저자/교신저자) J Korean Med Sci. 2011 May;26(5):625-30. • 2) Spectra of chromosomal aberrations in 325 leukemia patients and implications for the development of new molecular detection systems. (교신저자) J Korean Med Sci. 2011 Jul;26(7):886-92. • 3) Distinctive hematological abnormalities in East Asian neonates and children • with down syndrome. (주저자/교신저자) Int J Lab Hematol. 2011 ;33(4):369-377. • 4) Mitochondrial DNA copy number and hnRNP A2/B1 protein: biomarkers for direct exposure of benzene. (주저자/교신저자) Environ Toxicol Chem. 2011 Dec;30(12):2762-70. • 5) Donor cell origin of multiple myeloma occurring after allogeneic • haematopoietic stem cell transplantation in a patient with refractory anaemia with ring sideroblast. (주저자/교신저자) J Clin Pathol. 2011 Mar;64(3):265-8

11. 6) High-frequency minisatellite instability of the mitochondrial genome in • colorectal cancer tissue associated with clinicopathological values. • (주저자/교신저자) Int J Cancer. 2011 Nov 28. • 7) Paroxysmal nocturnal hemoglobinuria-myelodysplastic syndrome associated with marked thrombocytosis and acquired clonal cytogenetic abnormality of t(3;12)(q28;q15). (주저자/교신저자) Leukemia & Lymphoma. Accept • 공저자 • 1) Genetic relationship of fluconazole non-susceptible Candida glabrata isolates from nine Korean hospitals. (공저자) Mycoses. 2011 Sep;54(5):e659-62. • 2) Genetic Diversity among Korean Candida albicans Bloodstream Isolates: • Assessment by Multilocus Sequence Typing and Restriction Endonuclease • Analysis of Genomic DNA by Use of BssHII.(공저자) J Clin Microbiol. • 2011;49(7):2572-7. • Multilocus Sequence Typing for Candida albicans Isolates from Candidemic Patients: Comparison with Southern Blot Hybridization and Pulsed-field Gel Electrophoresis Analysis. (공저자) Korean J Lab Med. 2011;31(2):107-14

12. 8p11 myeloproliferative syndrome with BCR-FGFR1 rearrangement • presenting with T-lymphoblastic lymphoma and bone marrow stromal cell proliferation: A case report and review of the literature. (공저자) • Leuk Res. 2011 May;35(5):e30-4. • Engineering and Visualization of Bacteria for Targeting Infarcted • Myocardium. (공저자)Mol Ther. 2011 May;19(5):951-9. • 6) Genome-wide high density single-nucleotide polymorphism array-based karyotyping improves detection of clonal aberrations including der(9) deletion, but does not predict treatment outcomes after imatinib therapy in chronic myeloid leukemia. (공저자) Ann Hematol. 2011 Mar 8. [Epub ahead of print] • Treatment outcome of all-trans retinoic acid/anthracycline combination chemotherapy and the prognostic impact of FLT3/ITD mutation in acute promyelocytic leukemia patients. (공저자) Korean J Hematol. 2011 ;46(1):24-30 • Biofilm formation and genotyping of Candida haemulonii, Candida • pseudohaemulonii, and a proposed new species (Candida auris) isolates • from Korea. (공저자) Med Mycol. 2011 Jan;49(1):98-102

13. 9) Relationship between a hopeful attitude and cellular immunity in patients with breast cancer. (공저자) Gen Hosp sychiatry.  2011;33(4):371-6.  10) A novel a subtype allele that demonstrates allelic competition. (공저자) Ann Clin Lab Sci. 2011 ;41(3):282-4.

14. 2011 Manuscript preparation • Association of allelic expression imbalance of JAK2 V617F mutation and phenotypic diversity in MPN: • 김혜란/신명근 • 2) Prohibitin as a new biomarkers of leukemia for diagnosis, predicting prognosis, MRD monitoring and • molecular therapeutics: 김혜란/신명근 • Alteration of Mitochondrial Genome and its Pathophysiologial Role in Barrett’s Esophagus : • 김혜란/ 김환영/신명근 • 4) The spectrum of mitochondrial genome instability in acute myeloid leukemia and leukemia stem cells: • 김혜란/ 김환영/안애자/신명근 • Metformin selectively suppressed the proliferation of AML stem cells through increased phosphorylation • of AMP-activated protein kinase : 김혜란/ 엄하영/신명근 • 7) Serum fibrin degradation product is a valuable tumor marker for multiple myeloma: 김혜란/ 신명근 • 8) Mitochondrial minisatellite alterations in single AML stem cells associated with vicious cycle • hypothesis : 김혜란/ 김환영/신명근 • 9) Accuracy improvement for glucometer by reducing the hematocrit dependency using informatics • technology • 10) Mitochodrial Biomarkers for Benzopyrene Exposure

15. 2011 연구과제 수행 • 환경성 질환연구센터 3세부과제 • 한국연구재단 중견연구자사업 • 한국연구재단 해외우수연구소유치사업 2011 연구과제 프로포셜 • 보건산업진흥원 차세대유전체사업(센터사업)

16. 금성산성/천관산 /대둔산 산행 MD. Neal Young 2011 ASH (San Diego, CA)