1 / 1

A .

Additional File 5. A. Fold I κ B α expression (Normalized). IkB α ( 38 kDa). GAPDH (37 kDa). --. TNF α. TNF α. --. Control cells. WT-Ras cells. B. p65 (65 kDa). Total p65/GAPDH expression (Normalized). GAPDH (37 kDa). siRNA p65 (25nM). siRNA p65 (35nM). siRNA Control.

kevyn
Download Presentation

A .

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Additional File 5 A. Fold IκBαexpression (Normalized) IkBα (38 kDa) GAPDH (37 kDa) -- TNFα TNFα -- Control cells WT-Ras cells B. p65 (65 kDa) Total p65/GAPDH expression (Normalized) GAPDH (37 kDa) siRNA p65 (25nM) siRNA p65 (35nM) siRNA Control siRNAControl siRNAp65 (25nM) siRNAp65 (35nM) Control WT-Ras Additional File 5 IB levels in TNF-stimulated WT-Ras expressing cells, and p65 down-regulation by shRNAs to p65 (A) WT-Ras expressing MCF-7 cells were not-stimulated or stimulated by TNF (50 ng/ml). Activation of the NF-B pathway was analyzed by reduced levels of IB (=NF-B inhibitor), determined by WB. A representative experiment of n=3 is presented. (B) Validation of the p65-reducing activities of siRNAs to p65, determined by WB (Inhibition levels: 42% and 62% inhibition for 25 nM and 35 nM siRNA to p65, respectively). Reduction of p65 expression by siRNA p65 was denoted in n=3. -- -- TNFα TNFα

More Related