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Proteomics LIMS. Importance of Infrastructure. Abel Wolman and Linn Enslow perfect the Chlorination of Drinking Water. In one year, the death rate from typhoid dropped over 90% in the United States. LIMS. caLIMS Large LIMS built around Java RMI Inventory Control, Storage Location
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ProteomicsLIMS Fox Chase Cancer Center
Importance of Infrastructure Abel Wolman and Linn Enslow perfect the Chlorination of Drinking Water In one year, the death rate from typhoid dropped over 90% in the United States Fox Chase Cancer Center
LIMS • caLIMS • Large LIMS built around Java RMI • Inventory Control, Storage Location • Domain Specific Information • Need to add tables • Don’t want to mangle caLIMS structure Fox Chase Cancer Center
Sample Data Flow Fox Chase Cancer Center
Presentation Tier Business Tier Data Tier caLIMS + protLIMS tables Database protLIMS Struts framework (Servlets, JSP pages, HTML, XML) RMI JDBC caLIMS Logic Objects + protLIMSLogic Objects (Projects, samples, adapters etc) protLIMS EJB Data/File Access Objects Flat files FTP Proteomics LIMS Fox Chase Cancer Center
Added Tables Fox Chase Cancer Center
Prototype Interface Fox Chase Cancer Center
ProtLIMS keeps all caLIMS architectural features: N-Tiered structure Extensibility OS Portability Compatibility with other caLIMS systems ProtLIMS uses all major caLIMS technologies: Java Programming Language Java Server Pages, Java Servlets LDAP – Lightweight Directory Access Protocol Java RMI – Remote Method Invocation JDBC – Java Database Connectivity ProtLIMS also uses following technologies: Struts framework J2EE architecture approcah Design Principles Fox Chase Cancer Center
FGDP • Automated, multiple, simultaneous analyses of functional genomics data • Java based using RMI for distribution • Integrated through JDBC to microarray LIMS • Plan to use for QA/QC as well as data analysis for Proteomics LIMS Fox Chase Cancer Center
Models Data Flow Parsing/Image Analysis Normalization Statistical Analysis Filtering Pattern Recognition Image Generation Output Parsing Fox Chase Cancer Center